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dc.contributor.author
van der Weijden, Vera A.
dc.contributor.author
Schmidhauser, Meret
dc.contributor.author
Kurome, Mayuko
dc.contributor.author
Knubben, Johannes
dc.contributor.author
Flöter, Veronika L.
dc.contributor.author
Wolf, Eckhard
dc.contributor.author
Ulbrich, Susanne E.
dc.date.accessioned
2021-03-16T11:28:00Z
dc.date.available
2021-03-14T05:26:05Z
dc.date.available
2021-03-16T11:28:00Z
dc.date.issued
2021
dc.identifier.other
10.1186/s12864-021-07430-7
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/474340
dc.identifier.doi
10.3929/ethz-b-000474340
dc.description.abstract
Background The transcriptional changes around the time of embryonic genome activation in pre-implantation embryos indicate that this process is highly dynamic. In vitro produced porcine blastocysts are known to be less competent than in vivo developed blastocysts. To understand the conditions that compromise developmental competence of in vitro embryos, it is crucial to evaluate the transcriptional profile of porcine embryos during pre-implantation stages. In this study, we investigated the transcriptome dynamics in in vivo developed and in vitro produced 4-cell embryos, morulae and hatched blastocysts. Results In vivo developed and in vitro produced embryos displayed largely similar transcriptome profiles during development. Enriched canonical pathways from the 4-cell to the morula transition that were shared between in vivo developed and in vitro produced embryos included oxidative phosphorylation and EIF2 signaling. The shared canonical pathways from the morula to the hatched blastocyst transition were 14–3-3-mediated signaling, xenobiotic metabolism general signaling pathway, and NRF2-mediated oxidative stress response. The in vivo developed and in vitro produced hatched blastocysts further were compared to identify molecular signaling pathways indicative of lower developmental competence of in vitro produced hatched blastocysts. A higher metabolic rate and expression of the arginine transporter SLC7A1 were found in in vitro produced hatched blastocysts. Conclusions Our findings suggest that embryos with compromised developmental potential are arrested at an early stage of development, while embryos developing to the hatched blastocyst stage display largely similar transcriptome profiles, irrespective of the embryo source. The hatched blastocysts derived from the in vitro fertilization-pipeline showed an enrichment in molecular signaling pathways associated with lower developmental competence, compared to the in vivo developed embryos.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
BioMed Central
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
Transcriptomics
en_US
dc.subject
Porcine
en_US
dc.subject
Embryo development
en_US
dc.subject
In vivo embryo development
en_US
dc.subject
in vitro fertilization
en_US
dc.title
Transcriptome dynamics in early in vivo developing and in vitro produced porcine embryos
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2021-02-27
ethz.journal.title
BMC Genomics
ethz.journal.volume
22
en_US
ethz.journal.issue
1
en_US
ethz.pages.start
139
en_US
ethz.size
13 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.scopus
ethz.publication.place
London
en_US
ethz.publication.status
published
en_US
ethz.date.deposited
2021-03-14T05:26:21Z
ethz.source
SCOPUS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2021-03-16T11:28:19Z
ethz.rosetta.lastUpdated
2021-03-16T11:28:19Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
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