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dc.contributor.author
Otto, Lukas
dc.contributor.author
Wolint, Petra
dc.contributor.author
Bopp, Annina
dc.contributor.author
Woloszyk, Anna
dc.contributor.author
Becker, Anton S.
dc.contributor.author
Boss, Andreas
dc.contributor.author
Böni, Roland
dc.contributor.author
Calcagni, Maurizio
dc.contributor.author
Giovanoli, Pietro
dc.contributor.author
Hoerstrup, Simon P.
dc.contributor.author
Emmert, Maximilian Y.
dc.contributor.author
Buschmann, Johanna
dc.date.accessioned
2021-03-16T08:37:33Z
dc.date.available
2021-03-16T03:56:35Z
dc.date.available
2021-03-16T08:37:33Z
dc.date.issued
2021
dc.identifier.issn
2045-2322
dc.identifier.other
10.1038/s41598-021-84123-x
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/474722
dc.identifier.doi
10.3929/ethz-b-000474722
dc.description.abstract
Bone regeneration is a complex process and the clinical translation of tissue engineered constructs (TECs) remains a challenge. The combination of biomaterials and mesenchymal stem cells (MSCs) may enhance the healing process through paracrine effects. Here, we investigated the influence of cell format in combination with a collagen scaffold on key factors in bone healing process, such as mineralization, cell infiltration, vascularization, and ECM production. MSCs as single cells (2D-SCs), assembled into microtissues (3D-MTs) or their corresponding secretomes were combined with a collagen scaffold and incubated on the chicken embryo chorioallantoic membrane (CAM) for 7 days. A comprehensive quantitative analysis was performed on a cellular level by histology and by microcomputed tomography (microCT). In all experimental groups, accumulation of collagen and glycosaminoglycan within the scaffold was observed over time. A pronounced cell infiltration and vascularization from the interface to the surface region of the CAM was detected. The 3D-MT secretome showed a significant mineralization of the biomaterial using microCT compared to all other conditions. Furthermore, it revealed a homogeneous distribution pattern of mineralization deposits in contrast to the cell-based scaffolds, where mineralization was only at the surface. Therefore, the secretome of MSCs assembled into 3D-MTs may represent an interesting therapeutic strategy for a next-generation bone healing concept.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Nature
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.title
3D-microtissue derived secretome as a cell-free approach for enhanced mineralization of scaffolds in the chorioallantoic membrane model
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2021-03-08
ethz.journal.title
Scientific Reports
ethz.journal.volume
11
en_US
ethz.journal.issue
1
en_US
ethz.journal.abbreviated
Sci Rep
ethz.pages.start
5418
en_US
ethz.size
15 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
London
ethz.publication.status
published
en_US
ethz.date.deposited
2021-03-16T03:56:53Z
ethz.source
SCOPUS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2021-03-16T08:37:46Z
ethz.rosetta.lastUpdated
2024-02-02T13:18:49Z
ethz.rosetta.versionExported
true
ethz.COinS
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