Thermoplasmonic-Assisted Cyclic Cleavage Amplification for Self-Validating Plasmonic Detection of SARS-CoV-2

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Date
2021-04-27Type
- Journal Article
Citations
Cited 31 times in
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Cited 32 times in
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Abstract
The coronavirus disease 2019 (COVID-19) has penetrated every populated patch of the globe and sows destruction in our daily life. Reliable and sensitive virus sensing systems are therefore of vital importance for timely infection detection and transmission prevention. Here we present a thermoplasmonic-assisted dual-mode transducing (TP-DMT) concept, where an amplification-free-based direct viral RNA detection and an amplification-based cyclic fluorescence probe cleavage (CFPC) detection collaborated to provide a sensitive and self-validating plasmonic nanoplatform for quantifying trace amounts of SARS-CoV-2 within 30 min. In the CFPC detection, endonuclease IV recognized the synthetic abasic site and cleaved the fluorescent probes in the hybridized duplex. The nanoscale thermoplasmonic heating dehybridized the shortened fluorescent probes and facilitated the cyclical binding–cleavage–dissociation (BCD) process, which could deliver a highly sensitive amplification-based response. This TP-DMT approach was successfully validated by testing clinical COVID-19 patient samples, which indicated its potential applications in fast clinical infection screening and real-time environmental monitoring. Show more
Permanent link
https://doi.org/10.3929/ethz-b-000475512Publication status
publishedExternal links
Journal / series
ACS NanoVolume
Pages / Article No.
Publisher
American Chemical SocietySubject
Thermoplasmonics; SARS-CoV-2; COVID-19; Plasmonics; Biosensor; Cyclic cleavage amplificationOrganisational unit
03887 - Wang, Jing / Wang, Jing
Funding
198258 - Development of a real-time biosensing system of SARS-CoV-2 to improve healthcare workers safety during COVID 19 pandemics (SNF)
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Show all metadata
Citations
Cited 31 times in
Web of Science
Cited 32 times in
Scopus
ETH Bibliography
yes
Altmetrics