Show simple item record

dc.contributor.author
Bigini, Paolo
dc.contributor.author
Diana, Valentina
dc.contributor.author
Barbera, Sara
dc.contributor.author
Fumagalli, Elena
dc.contributor.author
Micotti, Edoardo
dc.contributor.author
Sitia, Leopoldo
dc.contributor.author
Paladini, Alessandra
dc.contributor.author
Bisighini, Cinzia
dc.contributor.author
De Grada, Laura
dc.contributor.author
Coloca, Laura
dc.contributor.author
Colombo, Laura
dc.contributor.author
Manca, Pina
dc.contributor.author
Bossolasco, Patrizia
dc.contributor.author
Malvestiti, Francesca
dc.contributor.author
Fiordaliso, Fabio
dc.contributor.author
Forloni, Gianluigi
dc.contributor.author
Morbidelli, Massimo
dc.contributor.author
Salmona, Mario
dc.contributor.author
Giardino, Daniela
dc.contributor.author
Mennini, Tiziana
dc.contributor.author
Moscatelli, Davide
dc.contributor.author
Silani, Vincenzo
dc.contributor.author
Cova, Lidia
dc.date.accessioned
2018-09-20T12:28:17Z
dc.date.available
2017-06-09T23:33:57Z
dc.date.available
2018-09-20T12:28:17Z
dc.date.issued
2012-02-27
dc.identifier.issn
1932-6203
dc.identifier.other
10.1371/journal.pone.0032326
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/48457
dc.identifier.doi
10.3929/ethz-b-000048457
dc.description.abstract
Stem Cell (SC) therapy is one of the most promising approaches for the treatment of Amyotrophic Lateral Sclerosis (ALS). Here we employed Super Paramagnetic Iron Oxide nanoparticles (SPIOn) and Hoechst 33258 to track human Amniotic Fluid Cells (hAFCs) after transplantation in the lateral ventricles of wobbler (a murine model of ALS) and healthy mice. By in vitro, in vivo and ex vivo approaches we found that: 1) the main physical parameters of SPIOn were maintained over time; 2) hAFCs efficiently internalized SPIOn into the cytoplasm while Hoechst 33258 labeled nuclei; 3) SPIOn internalization did not alter survival, cell cycle, proliferation, metabolism and phenotype of hAFCs; 4) after transplantation hAFCs rapidly spread to the whole ventricular system, but did not migrate into the brain parenchyma; 5) hAFCs survived for a long time in the ventricles of both wobbler and healthy mice; 6) the transplantation of double-labeled hAFCs did not influence mice survival.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Public Library of Science
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/3.0/
dc.title
Longitudinal Tracking of Human Fetal Cells Labeled with Super Paramagnetic Iron Oxide Nanoparticles in the Brain of Mice with Motor Neuron Disease
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 3.0 Unported
ethz.journal.title
PLoS ONE
ethz.journal.volume
7
en_US
ethz.journal.issue
2
en_US
ethz.journal.abbreviated
PLoS ONE
ethz.pages.start
e32326
en_US
ethz.size
12 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.nebis
006206116
ethz.publication.place
Lawrence, KS, USA
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02020 - Dep. Chemie und Angewandte Biowiss. / Dep. of Chemistry and Applied Biosc.::02516 - Inst. f. Chemie- und Bioingenieurwiss. / Inst. Chemical and Bioengineering::03451 - Morbidelli, Massimo (emeritus) / Morbidelli, Massimo (emeritus)
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02020 - Dep. Chemie und Angewandte Biowiss. / Dep. of Chemistry and Applied Biosc.::02516 - Inst. f. Chemie- und Bioingenieurwiss. / Inst. Chemical and Bioengineering::03451 - Morbidelli, Massimo (emeritus) / Morbidelli, Massimo (emeritus)
ethz.date.deposited
2017-06-09T23:34:48Z
ethz.source
ECIT
ethz.identifier.importid
imp59364f29e905c78474
ethz.ecitpid
pub:79755
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-14T12:06:19Z
ethz.rosetta.lastUpdated
2021-02-15T01:50:03Z
ethz.rosetta.versionExported
true
ethz.COinS
ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.atitle=Longitudinal%20Tracking%20of%20Human%20Fetal%20Cells%20Labeled%20with%20Super%20Paramagnetic%20Iron%20Oxide%20Nanoparticles%20in%20the%20Brain%20of%20Mice%20with%20Motor&rft.jtitle=PLoS%20ONE&rft.date=2012-02-27&rft.volume=7&rft.issue=2&rft.spage=e32326&rft.issn=1932-6203&rft.au=Bigini,%20Paolo&Diana,%20Valentina&Barbera,%20Sara&Fumagalli,%20Elena&Micotti,%20Edoardo&rft.genre=article&rft_id=info:doi/10.1371/journal.pone.0032326&
 Search print copy at ETH Library

Files in this item

Thumbnail

Publication type

Show simple item record