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dc.contributor.author
Incaviglia, Ilaria
dc.contributor.author
Frutiger, Andreas
dc.contributor.author
Blickenstorfer, Yves
dc.contributor.author
Treindl, Fridolin
dc.contributor.author
Ammirati, Giulia
dc.contributor.author
Lüchtefeld, Ines
dc.contributor.author
Dreier, Birgit
dc.contributor.author
Plückthun, Andreas
dc.contributor.author
Vörös, Janos
dc.contributor.author
Reichmuth, Andreas M.
dc.date.accessioned
2021-06-16T14:58:30Z
dc.date.available
2021-06-05T02:27:03Z
dc.date.available
2021-06-16T14:58:30Z
dc.date.issued
2021-04-23
dc.identifier.issn
2379-3694
dc.identifier.other
10.1021/acssensors.0c02480
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/488362
dc.description.abstract
In recent years, cell-based assays have been frequently used in molecular interaction analysis. Cell-based assays complement traditional biochemical and biophysical methods, as they allow for molecular interaction analysis, mode of action studies, and even drug screening processes to be performed under physiologically relevant conditions. In most cellular assays, biomolecules are usually labeled to achieve specificity. In order to overcome some of the drawbacks associated with label-based assays, we have recently introduced “cell-based molography” as a biosensor for the analysis of specific molecular interactions involving native membrane receptors in living cells. Here, we expand this assay to cytosolic protein–protein interactions. First, we created a biomimetic membrane receptor by tethering one cytosolic interaction partner to the plasma membrane. The artificial construct is then coherently arranged into a two-dimensional pattern within the cytosol of living cells. Thanks to the molographic sensor, the specific interactions between the coherently arranged protein and its endogenous interaction partners become visible in real time without the use of a fluorescent label. This method turns out to be an important extension of cell-based molography because it expands the range of interactions that can be analyzed by molography to those in the cytosol of living cells.
en_US
dc.language.iso
en
en_US
dc.publisher
American Chemical Society
en_US
dc.subject
cell-based assays
en_US
dc.subject
cell-based molography
en_US
dc.subject
protein-protein interactions
en_US
dc.subject
live-cell biosensors
en_US
dc.title
An Approach for the Real-Time Quantification of Cytosolic Protein–Protein Interactions in Living Cells
en_US
dc.type
Journal Article
dc.date.published
2021-03-24
ethz.journal.title
ACS Sensors
ethz.journal.volume
6
en_US
ethz.journal.issue
4
en_US
ethz.journal.abbreviated
ACS Sens
ethz.pages.start
1572
en_US
ethz.pages.end
1582
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
Washington, DC
en_US
ethz.publication.status
published
en_US
ethz.date.deposited
2021-06-05T02:27:10Z
ethz.source
SCOPUS
ethz.eth
yes
en_US
ethz.availability
Metadata only
en_US
ethz.rosetta.installDate
2021-06-16T14:58:38Z
ethz.rosetta.lastUpdated
2022-03-29T08:49:06Z
ethz.rosetta.versionExported
true
ethz.COinS
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