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dc.contributor.author
Burmeister, Alita R.
dc.contributor.author
Sullivan, Rachel M.
dc.contributor.author
Gallie, Jenna
dc.contributor.author
Lenski, Richard E.
dc.date.accessioned
2021-06-16T11:53:15Z
dc.date.available
2021-06-07T02:31:16Z
dc.date.available
2021-06-16T11:53:15Z
dc.date.issued
2021-05
dc.identifier.issn
1350-0872
dc.identifier.issn
1465-2080
dc.identifier.other
10.1099/mic.0.001063
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/488441
dc.identifier.doi
10.3929/ethz-b-000488441
dc.description.abstract
Bacteria often evolve resistance to phage through the loss or modification of cell surface receptors. In Escherichia coli and phage λ, such resistance can catalyze a coevolutionary arms race focused on host and phage structures that interact at the outer membrane. Here, we analyse another facet of this arms race involving interactions at the inner membrane, whereby E. coli evolves mutations in mannose permease-encoding genes manY and manZ that impair λ's ability to eject its DNA into the cytoplasm. We show that these man mutants arose concurrently with the arms race at the outer membrane. We tested the hypothesis that λ evolved an additional counter-defence that allowed them to infect bacteria with deleted man genes. The deletions severely impaired the ancestral λ, but some evolved phage grew well on the deletion mutants, indicating that they regained infectivity by evolving the ability to infect hosts independently of the mannose permease. This coevolutionary arms race fulfils the model of an inverse gene-for-gene infection network. Taken together, the interactions at both the outer and inner membranes reveal that coevolutionary arms races can be richer and more complex than is often appreciated.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Microbiology Society
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.title
Sustained coevolution of phage Lambda and Escherichia coli involves inner- as well as outer-membrane defences and counter-defences
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2021-05-25
ethz.journal.title
Microbiology
ethz.journal.volume
167
en_US
ethz.journal.issue
5
en_US
ethz.journal.abbreviated
Microbiology (N.Y.)
ethz.pages.start
001063
en_US
ethz.size
9 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.scopus
ethz.publication.place
London
en_US
ethz.publication.status
published
en_US
ethz.date.deposited
2021-06-07T02:31:33Z
ethz.source
SCOPUS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2021-06-16T11:53:25Z
ethz.rosetta.lastUpdated
2022-03-29T08:48:07Z
ethz.rosetta.versionExported
true
ethz.COinS
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