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dc.contributor.author
Muangsombut, Veerachat
dc.contributor.author
Withatanung, Patoo
dc.contributor.author
Chantratita, Narisara
dc.contributor.author
Chareonsudjai, Sorujsiri
dc.contributor.author
Lim, Jiali
dc.contributor.author
Galyov, Edouard E.
dc.contributor.author
Ottiwet, Orawan
dc.contributor.author
Sengyee, Sineenart
dc.contributor.author
Janesomboon, Sujintana
dc.contributor.author
Loessner, Martin J.
dc.contributor.author
Dunne, Matthew
dc.contributor.author
Korbsrisate, Sunee
dc.date.accessioned
2021-06-09T15:10:14Z
dc.date.available
2021-06-09T02:27:08Z
dc.date.available
2021-06-09T15:10:14Z
dc.date.issued
2021-05-26
dc.identifier.issn
0099-2240
dc.identifier.issn
1098-5336
dc.identifier.other
10.1128/AEM.03019-20
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/488887
dc.identifier.doi
10.3929/ethz-b-000488887
dc.description.abstract
Melioidosis is a life-threatening disease in humans caused by the Gram-negative bacterium Burkholderia pseudomallei. As severe septicemic melioidosis can lead to death within 24 to 48 h, a rapid diagnosis of melioidosis is critical for ensuring that an optimal antibiotic course is prescribed to patients. Here, we report the development and evaluation of a bacteriophage tail fiber-based latex agglutination assay for rapid detection of B. pseudomallei infection. Burkholderia phage E094 was isolated from rice paddy fields in northeast Thailand, and the whole genome was sequenced to identify its tail fiber (94TF). The 94TF complex was structurally characterized, which involved identification of a tail assembly protein that forms an essential component of the mature fiber. Recombinant 94TF was conjugated to latex beads and developed into an agglutination-based assay (94TF-LAA). 94TF-LAA was initially tested against a large library of Burkholderia and other bacterial strains before a field evaluation was performed during routine clinical testing. The sensitivity and specificity of the 94TF-LAA were assessed alongside standard biochemical analyses on 300 patient specimens collected from an area of melioidosis endemicity over 11 months. The 94TF-LAA took less than 5 min to produce positive agglutination, demonstrating 98% (95% confidence interval [CI] of 94.2% to 99.59%) sensitivity and 83% (95% CI of 75.64% to 88.35%) specificity compared to biochemical-based detection. Overall, we show how a Burkholderia-specific phage tail fiber can be exploited for rapid detection of B. pseudomallei. The 94TF-LAA has the potential for further development as a supplementary diagnostic to assist in clinical identification of this life-threatening pathogen. IMPORTANCE Rapid diagnosis of melioidosis is essential for ensuring that optimal antibiotic courses are prescribed to patients and thus warrants the development of cost-effective and easy-to-use tests for implementation in underresourced areas such as northeastern Thailand and other tropical regions. Phage tail fibers are an interesting alternative to antibodies for use in various diagnostic assays for different pathogenic bacteria. As exposed appendages of phages, tail fibers are physically robust and easy to manufacture, with many tail fibers (such as 94TF investigated here) capable of targeting a given bacterial species with remarkable specificity. Here, we demonstrate the effectiveness of a latex agglutination assay using a Burkholderia-specific tail fiber 94TF against biochemical-based detection methods that are the standard diagnostic in many areas where melioidosis is endemic.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
American Society for Microbiology
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
Burkholderia pseudomallei
en_US
dc.subject
Bacteriophage
en_US
dc.subject
Latex agglutination assay
en_US
dc.subject
Melioidosis
en_US
dc.subject
Rapid colony screening
en_US
dc.subject
Tail fiber
en_US
dc.subject
Tail assembly chaperone
en_US
dc.title
Rapid Clinical Screening of Burkholderia pseudomallei Colonies by a Bacteriophage Tail Fiber-Based Latex Agglutination Assay
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2021-04-02
ethz.journal.title
Applied and Environmental Microbiology
ethz.journal.volume
87
en_US
ethz.journal.issue
12
en_US
ethz.journal.abbreviated
Appl. Environ. Microbiol
ethz.pages.start
e0301920
en_US
ethz.size
16 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
Washington, DC
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02070 - Dep. Gesundheitswiss. und Technologie / Dep. of Health Sciences and Technology::02701 - Inst.f. Lebensmittelwiss.,Ernährung,Ges. / Institute of Food, Nutrition, and Health::03651 - Loessner, Martin / Loessner, Martin
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02070 - Dep. Gesundheitswiss. und Technologie / Dep. of Health Sciences and Technology::02701 - Inst.f. Lebensmittelwiss.,Ernährung,Ges. / Institute of Food, Nutrition, and Health::03651 - Loessner, Martin / Loessner, Martin
ethz.date.deposited
2021-06-09T02:27:21Z
ethz.source
SCOPUS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2021-06-09T15:10:22Z
ethz.rosetta.lastUpdated
2022-03-29T08:43:09Z
ethz.rosetta.versionExported
true
ethz.COinS
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