Structural basis of ribosomal frameshifting during translation of the SARS-CoV-2 RNA genome
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Date
2021-06-18Type
- Journal Article
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Cited 70 times in
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Cited 70 times in
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Abstract
Programmed ribosomal frameshifting is a key event during translation of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA genome that allows synthesis of the viral RNA-dependent RNA polymerase and downstream proteins. Here, we present the cryo–electron microscopy structure of a translating mammalian ribosome primed for frameshifting on the viral RNA. The viral RNA adopts a pseudoknot structure that lodges at the entry to the ribosomal messenger RNA (mRNA) channel to generate tension in the mRNA and promote frameshifting, whereas the nascent viral polyprotein forms distinct interactions with the ribosomal tunnel. Biochemical experiments validate the structural observations and reveal mechanistic and regulatory features that influence frameshifting efficiency. Finally, we compare compounds previously shown to reduce frameshifting with respect to their ability to inhibit SARS-CoV-2 replication, establishing coronavirus frameshifting as a target for antiviral intervention. Show more
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publishedExternal links
Journal / series
ScienceVolume
Pages / Article No.
Publisher
American Association for the Advancement of ScienceOrganisational unit
03861 - Bode, Jeffrey W. / Bode, Jeffrey W.
03556 - Ban, Nenad / Ban, Nenad
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Show all metadata
Citations
Cited 70 times in
Web of Science
Cited 70 times in
Scopus
ETH Bibliography
yes
Altmetrics