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dc.contributor.author
Brioudes, Florian
dc.contributor.author
Jay, Florence
dc.contributor.author
Sarazin, Alexis
dc.contributor.author
Grentzinger, Thomas
dc.contributor.author
Devers, Emanuel
dc.contributor.author
Voinnet, Olivier
dc.date.accessioned
2021-08-04T12:30:36Z
dc.date.available
2021-06-28T07:24:54Z
dc.date.available
2021-06-28T11:55:43Z
dc.date.available
2021-08-04T12:30:36Z
dc.date.issued
2021-08-02
dc.identifier.issn
0261-4189
dc.identifier.issn
1460-2075
dc.identifier.other
10.15252/embj.2020107455
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/491626
dc.identifier.doi
10.3929/ethz-b-000491626
dc.description.abstract
Plant microRNAs (miRNAs) guide cytosolic post-transcriptional gene silencing of sequence-complementary transcripts within the producing cells, as well as in distant cells and tissues. Here, we used an artificial miRNA-based system (amiRSUL) in Arabidopsis thaliana to explore the still elusive mechanisms of inter-cellular miRNA movement via forward genetics. This screen identified many mutant alleles of HASTY (HST), the ortholog of mammalian EXPORTIN5 (XPO5) with a recently reported role in miRNA biogenesis in Arabidopsis. In both epidermis-peeling and grafting assays, amiRSUL levels were reduced much more substantially in miRNA-recipient tissues than in silencing-emitting tissues. We ascribe this effect to HST controlling cell-to-cell and phloem-mediated movement of the processed amiRSUL, in addition to regulating its biogenesis. While HST is not required for the movement of free GFP or siRNAs, its cell-autonomous expression in amiRSUL-emitting tissues suffices to restore amiRSUL movement independently of its nucleo-cytosolic shuttling activity. By contrast, HST is dispensable for the movement and activity of amiRSUL within recipient tissues. Finally, HST enables movement of endogenous miRNAs that display mostly unaltered steady-state levels in hst mutant tissues. We discuss a role for HST as a hitherto unrecognized regulator of miRNA movement in relation to its recently assigned nuclear function at the nexus of MIRNA transcription and miRNA processing.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
EMBO Press
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
Arabidopsis
en_US
dc.subject
Argonaute
en_US
dc.subject
HASTY
en_US
dc.subject
miRNA
en_US
dc.subject
Movement
en_US
dc.title
HASTY, the Arabidopsis EXPORTIN5 ortholog, regulates cell-to-cell and vascular microRNA movement
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2021-06-21
ethz.journal.title
The EMBO Journal
ethz.journal.volume
40
en_US
ethz.journal.issue
15
en_US
ethz.journal.abbreviated
EMBO J
ethz.pages.start
e107455
en_US
ethz.size
22 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.grant
High resolution and chemical genetic approaches to RNA silencing mechanisms
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
Heidelberg
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02541 - Institut für Molekulare Pflanzenbiologie / Institute of Molecular Plant Biology::03876 - Voinnet, Olivier / Voinnet, Olivier
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02703 - Institut für Agrarwissenschaften / Institute of Agricultural Sciences::03970 - De Moraes, Consuelo / De Moraes, Consuelo
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02541 - Institut für Molekulare Pflanzenbiologie / Institute of Molecular Plant Biology::03876 - Voinnet, Olivier / Voinnet, Olivier
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02703 - Institut für Agrarwissenschaften / Institute of Agricultural Sciences::03970 - De Moraes, Consuelo / De Moraes, Consuelo
ethz.grant.agreementno
323071
ethz.grant.fundername
EC
ethz.grant.funderDoi
10.13039/501100000780
ethz.grant.program
FP7
ethz.date.deposited
2021-06-28T07:25:00Z
ethz.source
FORM
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2021-08-04T12:30:45Z
ethz.rosetta.lastUpdated
2022-03-29T10:55:43Z
ethz.rosetta.versionExported
true
ethz.COinS
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