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dc.contributor.author
Pasquariello, Rolando
dc.contributor.author
Verdile, Nicole
dc.contributor.author
Pavlovic, Radmila
dc.contributor.author
Panseri, Sara
dc.contributor.author
Schirmer, Kristin
dc.contributor.author
Brevini, Tiziana A.L.
dc.contributor.author
Gandolfi, Fulvio
dc.date.accessioned
2021-07-13T13:17:53Z
dc.date.available
2021-07-05T02:54:27Z
dc.date.available
2021-07-13T13:17:53Z
dc.date.issued
2021-06
dc.identifier.issn
2073-4409
dc.identifier.other
10.3390/cells10061555
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/492908
dc.identifier.doi
10.3929/ethz-b-000492908
dc.description.abstract
We derived two novel cell lines from rainbow trout (RT) proximal (RTpi-MI) and distal intestine (RTdi-MI) and compared them with the previously established continuous cell line RTgutGC. Intestinal stem cells, differentiating and differentiated epithelial cells, and connective cells were found in all cell lines. The cell lines formed a polarized barrier, which was not permeable to large molecules and absorbed proline and glucose. High seeding density induced their differentiation into more mature phenotypes, as indicated by the downregulation of intestinal stem cell-related genes (i.e., sox9, hopx and lgr5), whereas alkaline phosphatase activity was upregulated. Other enterocyte markers (i.e., sglt1 and pept1), however, were not regulated as expected. In all cell lines, the presence of a mixed population of epithelial and stromal cells was characterized for the first time. The expression by the stromal component of lgr5, a stem cell niche regulatory molecule, may explain why these lines proliferate stably in vitro. Although most parameters were conserved among the three cell lines, some significant differences were observed, suggesting that characteristics typical of each tract are partly conserved in vitro as well.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
MDPI
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
fish
en_US
dc.subject
intestinal cell lines
en_US
dc.subject
intestinal stem cells
en_US
dc.subject
enterocyte differentiation markers
en_US
dc.subject
intestinal barrier in vitro
en_US
dc.subject
cell differentiation
en_US
dc.subject
nutrition
en_US
dc.title
New Stable Cell Lines Derived from the Proximal and Distal Intestine of Rainbow Trout (Oncorhynchus mykiss) Retain Several Properties Observed In Vivo
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2021-06-19
ethz.journal.title
Cells
ethz.journal.volume
10
en_US
ethz.journal.issue
6
en_US
ethz.pages.start
1555
en_US
ethz.size
17 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
Basel
en_US
ethz.publication.status
published
en_US
ethz.date.deposited
2021-07-05T02:54:50Z
ethz.source
WOS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2021-07-13T13:17:59Z
ethz.rosetta.lastUpdated
2022-03-29T10:23:34Z
ethz.rosetta.versionExported
true
ethz.COinS
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