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dc.contributor.author
Wieloch, Thomas
dc.contributor.author
Werner, Roland Anton
dc.contributor.author
Schleucher, Jürgen
dc.date.accessioned
2021-07-08T09:23:23Z
dc.date.available
2021-07-07T11:40:19Z
dc.date.available
2021-07-08T09:23:23Z
dc.date.issued
2021
dc.identifier.issn
1460-2431
dc.identifier.issn
0022-0957
dc.identifier.other
10.1093/jxb/erab316
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/493415
dc.description.abstract
Within the plant and earth sciences, stable isotope analysis is a versatile tool conveying information (inter alia) about plant physiological and paleoclimate variability across scales. Here, we identify a 13C signal (i.e., systematic 13C/ 12C variation) at tree-ring glucose C-4 and report an experimentally testable theory on its origin. We propose the signal is introduced by glyceraldehyde-3-phosphate dehydrogenases in the cytosol of leaves. It conveys two kinds of (potentially convoluted) information: (i) commitment of glyceraldehyde 3-phosphate to 3-phosphoglycerate vs. fructose 1,6-bisphosphate metabolism, and (ii) the contribution of non-phosphorylating vs. phosphorylating glyceraldehyde-3-phosphate dehydrogenase to catalysing the glyceraldehyde 3-phosphate to 3-phosphoglycerate forward reaction of glycolysis. Theory is supported by 13C fractionation modelling. Modelling results provide first evidence in support of the cytosolic oxidation-reduction (COR) cycle, a carbon-neutral mechanism supplying NADPH at the expense of ATP and NADH which may help to maintain leaf-cytosolic redox balances. In line with expectations related to COR cycling, we found a positive correlation between air vapour pressure deficit and 13C discrimination at glucose C-4. Overall, 13C-4 signal analysis may enable an improved understanding of leaf carbon and energy metabolism.
en_US
dc.language.iso
en
en_US
dc.publisher
Oxford University Press
en_US
dc.subject
Carbon allocation
en_US
dc.subject
Carbon flux
en_US
dc.subject
Carbon stable isotopes
en_US
dc.subject
COR cycle
en_US
dc.subject
Cytosolic oxidationreduction cycle
en_US
dc.subject
Energy metabolism
en_US
dc.subject
Glyceraldehyde-3-phosphate dehydrogenase
en_US
dc.subject
Intramolecular isotope analysis
en_US
dc.subject
Isotope fractionation model
en_US
dc.subject
Primary carbon metabolism
en_US
dc.title
Carbon flux around leaf-cytosolic glyceraldehyde-3-phosphate dehydrogenase introduces a 13C signal in plant glucose
en_US
dc.type
Journal Article
dc.date.published
2021-07-05
ethz.journal.title
Journal of Experimental Botany
ethz.journal.abbreviated
J. Exp. Bot.
ethz.size
24 p.
en_US
ethz.publication.place
Oxford
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02703 - Institut für Agrarwissenschaften / Institute of Agricultural Sciences::03648 - Buchmann, Nina / Buchmann, Nina
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02703 - Institut für Agrarwissenschaften / Institute of Agricultural Sciences::03648 - Buchmann, Nina / Buchmann, Nina
en_US
ethz.date.deposited
2021-07-07T11:40:28Z
ethz.source
FORM
ethz.eth
yes
en_US
ethz.availability
Metadata only
en_US
ethz.rosetta.exportRequired
true
ethz.COinS
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