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dc.contributor.author
Paganini, Carolina
dc.contributor.author
Hettich, Britta
dc.contributor.author
Kopp, Marie R.G.
dc.contributor.author
Eördögh, Adam
dc.contributor.author
Capasso Palmiero, Umberto
dc.contributor.author
Adamo, Giorgia
dc.contributor.author
Touzet, Nicolas
dc.contributor.author
Manno, Mauro
dc.contributor.author
Bongiovanni, Antonella
dc.contributor.author
Rivera Fuentes, Pablo
dc.contributor.author
Leroux, Jean-Christophe
dc.contributor.author
Arosio, Paolo
dc.date.accessioned
2022-03-04T13:05:01Z
dc.date.available
2021-07-09T11:29:49Z
dc.date.available
2021-07-09T11:31:09Z
dc.date.available
2022-03-04T13:05:01Z
dc.date.issued
2022-03-02
dc.identifier.issn
2192-2640
dc.identifier.issn
2192-2659
dc.identifier.other
10.1002/adhm.202100021
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/493765
dc.identifier.doi
10.3929/ethz-b-000493765
dc.description.abstract
Extracellular vesicles (EVs) are emerging as promising diagnostic and therapeutic tools for a variety of diseases. The characterization of EVs requires a series of orthogonal techniques that are overall time- and material-consuming. Here, a microfluidic device is presented that exploits the combination of diffusion sizing and multiwavelength fluorescence detection to simultaneously provide information on EV size, concentration, and composition. The latter is achieved with the nonspecific staining of lipids and proteins combined with the specific staining of EV markers such as EV-associated tetraspanins via antibodies. The device can be operated as a single-step immunoassay thanks to the integrated separation and quantification of free and EV-bound fluorophores. This microfluidic technique is capable of detecting and quantifying components associated to EV subtypes and impurities and thus to measure EV purity in a time scale of minutes, requiring less than 5 µL of sample and minimal sample handling before the analysis. Moreover, the analysis is performed directly in solution without immobilization steps. Therefore, this method can accelerate screening of EV samples and aid the evaluation of sample reproducibility, representing an important complementary tool to the current array of biophysical methods for EV characterization, particularly valuable for instance for bioprocess development.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Wiley-VCH
en_US
dc.rights.uri
http://creativecommons.org/licenses/by-nc/4.0/
dc.subject
characterization
en_US
dc.subject
extracellular vesicles
en_US
dc.subject
fluorescence
en_US
dc.subject
immunoassay
en_US
dc.subject
microfluidics
en_US
dc.subject
purity
en_US
dc.subject
quantification
en_US
dc.title
Rapid Characterization and Quantification of Extracellular Vesicles by Fluorescence‐Based Microfluidic Diffusion Sizing
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution-NonCommercial 4.0 International
dc.date.published
2021-06-10
ethz.journal.title
Advanced Healthcare Materials
ethz.journal.volume
11
en_US
ethz.journal.issue
5
en_US
ethz.journal.abbreviated
Adv. Healthcare Mater.
ethz.pages.start
2100021
en_US
ethz.size
13 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.grant
Extracellular vesicles from a natural source for tailor-made nanomaterials
en_US
ethz.grant
Microfluidic assays for the quantification of low concentrations of proteins in heterogeneous mixtures
en_US
ethz.grant
Intradermal delivery of stem cell exosomes
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
Weinheim
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02020 - Dep. Chemie und Angewandte Biowiss. / Dep. of Chemistry and Applied Biosc.::02516 - Inst. f. Chemie- und Bioingenieurwiss. / Inst. Chemical and Bioengineering::09572 - Arosio, Paolo / Arosio, Paolo
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02020 - Dep. Chemie und Angewandte Biowiss. / Dep. of Chemistry and Applied Biosc.::02534 - Institut für Pharmazeutische Wiss. / Institute of Pharmaceutical Sciences::03811 - Leroux, Jean-Christophe / Leroux, Jean-Christophe
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02020 - Dep. Chemie und Angewandte Biowiss. / Dep. of Chemistry and Applied Biosc.::02534 - Institut für Pharmazeutische Wiss. / Institute of Pharmaceutical Sciences::03811 - Leroux, Jean-Christophe / Leroux, Jean-Christophe
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02020 - Dep. Chemie und Angewandte Biowiss. / Dep. of Chemistry and Applied Biosc.::02516 - Inst. f. Chemie- und Bioingenieurwiss. / Inst. Chemical and Bioengineering::09572 - Arosio, Paolo / Arosio, Paolo
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02020 - Dep. Chemie und Angewandte Biowiss. / Dep. of Chemistry and Applied Biosc.::02534 - Institut für Pharmazeutische Wiss. / Institute of Pharmaceutical Sciences::03811 - Leroux, Jean-Christophe / Leroux, Jean-Christophe
en_US
ethz.grant.agreementno
801338
ethz.grant.agreementno
ETH-33 17-2
ethz.grant.agreementno
ETH-10 16-1
ethz.grant.agreementno
801338
ethz.grant.agreementno
ETH-33 17-2
ethz.grant.agreementno
ETH-10 16-1
ethz.grant.fundername
EC
ethz.grant.fundername
ETHZ
ethz.grant.fundername
ETHZ
ethz.grant.fundername
EC
ethz.grant.fundername
ETHZ
ethz.grant.fundername
ETHZ
ethz.grant.funderDoi
10.13039/501100000780
ethz.grant.funderDoi
10.13039/501100003006
ethz.grant.funderDoi
10.13039/501100003006
ethz.grant.funderDoi
10.13039/501100000780
ethz.grant.funderDoi
10.13039/501100003006
ethz.grant.funderDoi
10.13039/501100003006
ethz.grant.program
H2020
ethz.grant.program
ETH Grants
ethz.grant.program
ETH Grants
ethz.grant.program
H2020
ethz.grant.program
ETH Grants
ethz.grant.program
ETH Grants
ethz.date.deposited
2021-07-09T11:29:59Z
ethz.source
FORM
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2022-03-04T13:05:08Z
ethz.rosetta.lastUpdated
2024-02-02T16:28:10Z
ethz.rosetta.versionExported
true
ethz.COinS
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