Osmotic Treatment for Quantifying Cell Wall Elasticity in the Sepal of Arabidopsis thaliana
dc.contributor.author
Sapala, Aleksandra
dc.contributor.author
Smith, Richard S.
dc.contributor.editor
Naseem, Muhammad N.
dc.contributor.editor
Dandekar, Thomas
dc.date.accessioned
2021-08-06T08:52:14Z
dc.date.available
2021-07-15T10:22:37Z
dc.date.available
2021-08-06T08:26:09Z
dc.date.available
2021-08-06T08:36:05Z
dc.date.available
2021-08-06T08:48:39Z
dc.date.available
2021-08-06T08:52:14Z
dc.date.issued
2020
dc.identifier.isbn
978-1-0716-0183-9
en_US
dc.identifier.isbn
978-1-0716-0182-2
en_US
dc.identifier.isbn
978-1-0716-0185-3
en_US
dc.identifier.issn
1064-3745
dc.identifier.issn
1940-6029
dc.identifier.other
10.1007/978-1-0716-0183-9_11
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/494819
dc.description.abstract
Elastic properties of the cell wall play a key role in regulating plant growth and morphogenesis; however, measuring them in vivo remains a challenge. Although several new methods have recently become available, they all have substantial drawbacks. Here we describe a detailed protocol for osmotic treatments, which is based on the idea of releasing the turgor pressure within the cell and measuring the resulting deformation. When placed in hyperosmotic solution, cells lose water via osmosis and shrink. Confocal images of the tissue, taken before and after this treatment, are quantified using high-resolution surface projections in MorphoGraphX. The cell shrinkage observed can then be used to estimate cell wall elasticity. This allows qualitative comparisons of cell wall properties within organs or between genotypes and can be combined with mechanical simulations to give quantitative estimates of the cells’ Young’s moduli. We use the abaxial sepal of Arabidopsis thaliana as an easily accessible model system to present our approach, but it can potentially be used on many other plant organs. The main challenges of this technique are choosing the optimal concentration of the hyperosmotic solution and producing high-quality confocal images (with cell walls visualized) good enough for segmentation in MorphoGraphX. © 2020 Springer Nature Switzerland AG
en_US
dc.language.iso
en
en_US
dc.publisher
Humana Press
en_US
dc.title
Osmotic Treatment for Quantifying Cell Wall Elasticity in the Sepal of Arabidopsis thaliana
en_US
dc.type
Book Chapter
dc.date.published
2019-12-04
ethz.book.title
Plant Stem Cells: Methods and Protocols
en_US
ethz.journal.title
Methods in Molecular Biology
ethz.journal.volume
2094
en_US
ethz.journal.abbreviated
Methods Mol Biol
ethz.pages.start
101
en_US
ethz.pages.end
112
en_US
ethz.identifier.wos
ethz.publication.place
New York, NY
en_US
ethz.publication.status
published
en_US
ethz.date.deposited
2021-07-15T10:23:53Z
ethz.source
WOS
ethz.eth
yes
en_US
ethz.availability
Metadata only
en_US
ethz.rosetta.installDate
2021-08-06T08:48:46Z
ethz.rosetta.lastUpdated
2022-03-29T10:58:17Z
ethz.rosetta.versionExported
true
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Book Chapter [9513]