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dc.contributor.author
Grey, Erin K.
dc.contributor.author
Bernatchez, Louis
dc.contributor.author
Cassey, Phillip
dc.contributor.author
Deiner, Kristy
dc.contributor.author
Deveney, Marty
dc.contributor.author
Howland, Kimberly L.
dc.contributor.author
Lacoursière‐Roussel, Anaïs
dc.contributor.author
Leong, Sandric C.Y.
dc.contributor.author
Li, Yiyuan
dc.contributor.author
Olds, Brett
dc.contributor.author
Pfrender, Michael E.
dc.contributor.author
Prowse, Thomas A.A.
dc.contributor.author
Renshaw, Mark A.
dc.contributor.author
Lodge, David M.
dc.date.accessioned
2021-07-23T12:50:23Z
dc.date.available
2021-07-21T08:26:02Z
dc.date.available
2021-07-23T12:48:34Z
dc.date.available
2021-07-23T12:50:23Z
dc.date.issued
2018-06-11
dc.identifier.issn
2045-2322
dc.identifier.other
10.1038/s41598-018-27048-2
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/496741
dc.identifier.doi
10.3929/ethz-b-000496741
dc.description.abstract
Environmental DNA (eDNA) metabarcoding can greatly enhance our understanding of global biodiversity and our ability to detect rare or cryptic species. However, sampling effort must be considered when interpreting results from these surveys. We explored how sampling effort influenced biodiversity patterns and nonindigenous species (NIS) detection in an eDNA metabarcoding survey of four commercial ports. Overall, we captured sequences from 18 metazoan phyla with minimal differences in taxonomic coverage between 18 S and COI primer sets. While community dissimilarity patterns were consistent across primers and sampling effort, richness patterns were not, suggesting that richness estimates are extremely sensitive to primer choice and sampling effort. The survey detected 64 potential NIS, with COI identifying more known NIS from port checklists but 18 S identifying more operational taxonomic units shared between three or more ports that represent un-recorded potential NIS. Overall, we conclude that eDNA metabarcoding surveys can reveal global similarity patterns among ports across a broad array of taxa and can also detect potential NIS in these key habitats. However, richness estimates and species assignments require caution. Based on results of this study, we make several recommendations for port eDNA sampling design and suggest several areas for future research.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Macmillan Publishers
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.title
Effects of sampling effort on biodiversity patterns estimated from environmental DNA metabarcoding surveys
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
ethz.journal.title
Scientific Reports
ethz.journal.volume
8
en_US
ethz.journal.abbreviated
Sci Rep
ethz.pages.start
8843
en_US
ethz.size
10 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.publication.place
London
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02721 - Inst. f. Biogeochemie u. Schadstoffdyn. / Inst. Biogeochem. and Pollutant Dynamics::09719 - Deiner, Kristy / Deiner, Kristy
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02721 - Inst. f. Biogeochemie u. Schadstoffdyn. / Inst. Biogeochem. and Pollutant Dynamics::09719 - Deiner, Kristy / Deiner, Kristy
en_US
ethz.relation.isReferencedBy
10.1038/s41598-018-27048-2
ethz.date.deposited
2021-07-21T08:26:07Z
ethz.source
FORM
ethz.eth
no
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2021-07-23T12:48:44Z
ethz.rosetta.lastUpdated
2022-03-29T10:38:02Z
ethz.rosetta.versionExported
true
ethz.COinS
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