Show simple item record

dc.contributor.author
Ni, Ruiqing
dc.contributor.author
Villois, Alessia
dc.contributor.author
Deán-Ben, Xosé-Luís
dc.contributor.author
Chen, Zhenyue
dc.contributor.author
Vaas, Markus
dc.contributor.author
Stavrakis, Stavros
dc.contributor.author
Shi, Gloria
dc.contributor.author
de Mello, Andrew
dc.contributor.author
Razansky, Daniel
dc.contributor.author
Arosio, Paolo
dc.contributor.author
Klohs, Jan
dc.date.accessioned
2021-07-29T12:47:21Z
dc.date.available
2021-07-29T03:31:14Z
dc.date.available
2021-07-29T12:47:21Z
dc.date.issued
2021-09
dc.identifier.other
10.1016/j.pacs.2021.100285
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/498282
dc.identifier.doi
10.3929/ethz-b-000498282
dc.description.abstract
The abnormal deposition of fibrillar beta-amyloid (Aβ) deposits in the brain is one of the major histopathological hallmarks of Alzheimer’s disease (AD). Here, we characterized curcumin-derivative CRANAD-2 for multi-spectral optoacoustic tomography and fluorescence imaging of brain Aβ deposits in the arcAβ mouse model of AD cerebral amyloidosis. CRANAD-2 showed a specific and quantitative detection of Aβ fibrils in vitro, even in complex mixtures, and it is capable of distinguishing between monomeric and fibrillar forms of Aβ. In vivo epi-fluorescence microscopy and optoacoustic tomography after intravenous CRANAD-2 administration demonstrated higher cortical retention in arcAβ compared to non-transgenic littermate mice. Immunohistochemistry showed co-localization of CRANAD-2 and Aβ deposits in arcAβ mouse brain sections, thus verifying the specificity of the probe. In conclusion, we demonstrate suitability of CRANAD-2 for optical detection of Aβ deposits in animal models of AD pathology, which facilitates mechanistic studies and the monitoring of putative treatments targeting Aβ deposits.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Elsevier
en_US
dc.rights.uri
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject
Alzheimer’s disease
en_US
dc.subject
Amyloid-beta
en_US
dc.subject
Animal model
en_US
dc.subject
Fluorescence imaging
en_US
dc.subject
Multi-spectral optoacoustic tomography
en_US
dc.title
In-vitro and in-vivo characterization of CRANAD-2 for multi-spectral optoacoustic tomography and fluorescence imaging of amyloid-beta deposits in Alzheimer mice
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
dc.date.published
2021-07-15
ethz.journal.title
Photoacoustics
ethz.journal.volume
23
en_US
ethz.pages.start
100285
en_US
ethz.size
12 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
Amsterdam
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02020 - Dep. Chemie und Angewandte Biowiss. / Dep. of Chemistry and Applied Biosc.::02516 - Inst. f. Chemie- und Bioingenieurwiss. / Inst. Chemical and Bioengineering::03914 - deMello, Andrew / deMello, Andrew
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02020 - Dep. Chemie und Angewandte Biowiss. / Dep. of Chemistry and Applied Biosc.::02516 - Inst. f. Chemie- und Bioingenieurwiss. / Inst. Chemical and Bioengineering::09572 - Arosio, Paolo / Arosio, Paolo
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02140 - Dep. Inf.technologie und Elektrotechnik / Dep. of Inform.Technol. Electrical Eng.::02631 - Institut für Biomedizinische Technik / Institute for Biomedical Engineering::09648 - Razansky, Daniel / Razansky, Daniel
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02020 - Dep. Chemie und Angewandte Biowiss. / Dep. of Chemistry and Applied Biosc.::02516 - Inst. f. Chemie- und Bioingenieurwiss. / Inst. Chemical and Bioengineering::03914 - deMello, Andrew / deMello, Andrew
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02020 - Dep. Chemie und Angewandte Biowiss. / Dep. of Chemistry and Applied Biosc.::02516 - Inst. f. Chemie- und Bioingenieurwiss. / Inst. Chemical and Bioengineering::09572 - Arosio, Paolo / Arosio, Paolo
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02140 - Dep. Inf.technologie und Elektrotechnik / Dep. of Inform.Technol. Electrical Eng.::02631 - Institut für Biomedizinische Technik / Institute for Biomedical Engineering::09648 - Razansky, Daniel / Razansky, Daniel
ethz.date.deposited
2021-07-29T03:31:17Z
ethz.source
SCOPUS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2021-07-29T12:47:28Z
ethz.rosetta.lastUpdated
2022-03-29T10:48:45Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.atitle=In-vitro%20and%20in-vivo%20characterization%20of%20CRANAD-2%20for%20multi-spectral%20optoacoustic%20tomography%20and%20fluorescence%20imaging%20of%20amyloid-beta%20depos&rft.jtitle=Photoacoustics&rft.date=2021-09&rft.volume=23&rft.spage=100285&rft.au=Ni,%20Ruiqing&Villois,%20Alessia&De%C3%A1n-Ben,%20Xos%C3%A9-Lu%C3%ADs&Chen,%20Zhenyue&Vaas,%20Markus&rft.genre=article&rft_id=info:doi/10.1016/j.pacs.2021.100285&
 Search print copy at ETH Library

Files in this item

Thumbnail

Publication type

Show simple item record