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dc.contributor.author
Oberhänsli, Thomas
dc.contributor.author
Dalbosco, Anna
dc.contributor.author
Leschenne, Virginie
dc.contributor.author
Bohr, Anne
dc.contributor.author
Buchleither, Sascha
dc.contributor.author
Tamm, Lucius
dc.contributor.author
Wille, Lukas
dc.contributor.author
Aćimović, Srđan G.
dc.contributor.author
Khodadadi, Fatemeh
dc.contributor.author
Ryu, Young-Hyun
dc.contributor.author
Studer, Bruno
dc.contributor.author
Schärer, Hans-Jakob
dc.contributor.author
Patocchi, Andrea
dc.date.accessioned
2021-08-02T05:43:27Z
dc.date.available
2021-08-01T15:14:12Z
dc.date.available
2021-08-02T05:43:27Z
dc.date.issued
2021-05-29
dc.identifier.issn
2662-4044
dc.identifier.other
10.1186/s43170-021-00039-6
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/498811
dc.identifier.doi
10.3929/ethz-b-000498811
dc.description.abstract
Background Apple blotch (AB) caused by Diplocarpon coronariae (Dc) has been established in Europe since 2010. AB is a serious apple disease, mostly in low input orchards and in cider production areas in Northern Italy, Switzerland, Austria and Germany. However, the epidemiology and population genetic structure of this pathogen is unknown. Methods We developed twelve Dc-specific microsatellite markers and screened DNA of both pure fungal isolates and infected apple leaves. The marker data of 313 European samples of Dc were compared to Dc isolates from Asia (n = 7) and the USA (n = 3). Results We found 31 distinct multilocus genotypes (MLGs) in European samples, and seven additional MLGs in the Asian and USA samples. The European samples had the typical genetic signature of a recently introduced species including high clonality, a low number of private alleles and one dominant MLG across all the sampling sites. All European MLGs were genetically distant from those MLGs of Asian and USA origin. Based on the lack of linkage disequilibrium observed, there is evidence that Dc undergoes regular cycles of sexual recombination in the European population, although the sexual stage (apothecia) has not been observed in Europe. Conclusions The twelve newly developed SSR markers reported here provide a useful tool to characterize the population genetic diversity and structure of Dc in Europe. Our study supports the hypothesis that Dc is a recently introduced pathogen in Europe, but of currently unknown origin. Dc has a large effective population size during field epidemics, so we believe that the pathogen has substantial evolutionary potential. Application of the SSR markers to large-scale and diverse Dc samples will help to better understand the epidemiology of AB, which has become a global apple disease, and will help guide effective mitigation strategies based on disease management and resistance breeding.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
BioMed Central
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
Apple blotch
en_US
dc.subject
Diplocarpon coronariae syn. Marssonina coronaria
en_US
dc.subject
Microsatellite markers
en_US
dc.subject
Clonal populations
en_US
dc.subject
Mixed reproduction
en_US
dc.title
Multiplexed SSR marker analysis of Diplocarpon coronariae reveals clonality within samples from Middle Europe and genetic distance from Asian and North American isolates
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
ethz.journal.title
CABI Agriculture and Bioscience
ethz.journal.volume
2
en_US
ethz.pages.start
21
en_US
ethz.size
15 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.publication.place
London
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02703 - Institut für Agrarwissenschaften / Institute of Agricultural Sciences::03969 - Studer, Bruno / Studer, Bruno
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02703 - Institut für Agrarwissenschaften / Institute of Agricultural Sciences::03969 - Studer, Bruno / Studer, Bruno
en_US
ethz.date.deposited
2021-08-01T15:14:23Z
ethz.source
FORM
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2021-08-02T05:43:33Z
ethz.rosetta.lastUpdated
2022-03-29T10:51:40Z
ethz.rosetta.versionExported
true
ethz.COinS
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