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dc.contributor.author
Lacoursière‐Roussel, Anaïs
dc.contributor.author
Howland, Kimberly
dc.contributor.author
Normandeau, Eric
dc.contributor.author
Grey, Erin K.
dc.contributor.author
Archambault, Philippe
dc.contributor.author
Deiner, Kristy
dc.contributor.author
Lodge, David M.
dc.contributor.author
Hernandez, Cecilia
dc.contributor.author
Leduc, Noémie
dc.contributor.author
Bernatchez, Louis
dc.date.accessioned
2021-08-26T11:45:46Z
dc.date.available
2021-08-26T09:45:07Z
dc.date.available
2021-08-26T11:45:46Z
dc.date.issued
2018-08
dc.identifier.issn
2045-7758
dc.identifier.other
10.1002/ece3.4213
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/502270
dc.identifier.doi
10.3929/ethz-b-000502270
dc.description.abstract
Because significant global changes are currently underway in the Arctic, creating a large-scale standardized database for Arctic marine biodiversity is particularly pressing. This study evaluates the potential of aquatic environmental DNA (eDNA) metabarcoding to detect Arctic coastal biodiversity changes and characterizes the local spatio-temporal distribution of eDNA in two locations. We extracted and amplified eDNA using two COI primer pairs from ~80 water samples that were collected across two Canadian Arctic ports, Churchill and Iqaluit, based on optimized sampling and preservation methods for remote regions surveys. Results demonstrate that aquatic eDNA surveys have the potential to document large-scale Arctic biodiversity change by providing a rapid overview of coastal metazoan biodiversity, detecting nonindigenous species, and allowing sampling in both open water and under the ice cover by local northern-based communities. We show that DNA sequences of ~50% of known Canadian Arctic species and potential invaders are currently present in public databases. A similar proportion of operational taxonomic units was identified at the species level with eDNA metabarcoding, for a total of 181 species identified at both sites. Despite the cold and well-mixed coastal environment, species composition was vertically heterogeneous, in part due to river inflow in the estuarine ecosystem, and differed between the water column and tide pools. Thus, COI-based eDNA metabarcoding may quickly improve large-scale Arctic biomonitoring using eDNA, but we caution that aquatic eDNA sampling needs to be standardized over space and time to accurately evaluate community structure changes.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Wiley
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.title
eDNA metabarcoding as a new surveillance approach for coastal Arctic biodiversity
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2018-07-13
ethz.journal.title
Ecology and Evolution
ethz.journal.volume
8
en_US
ethz.journal.issue
16
en_US
ethz.journal.abbreviated
Ecol Evol
ethz.pages.start
7763
en_US
ethz.pages.end
7777
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.publication.place
Oxford
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02721 - Inst. f. Biogeochemie u. Schadstoffdyn. / Inst. Biogeochem. and Pollutant Dynamics::09719 - Deiner, Kristy / Deiner, Kristy
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02721 - Inst. f. Biogeochemie u. Schadstoffdyn. / Inst. Biogeochem. and Pollutant Dynamics::09719 - Deiner, Kristy / Deiner, Kristy
en_US
ethz.date.deposited
2021-08-26T09:45:13Z
ethz.source
FORM
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2021-08-26T11:45:54Z
ethz.rosetta.lastUpdated
2022-03-29T11:19:37Z
ethz.rosetta.versionExported
true
ethz.COinS
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