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dc.contributor.author
Miranda, Camila O.
dc.contributor.author
Hegedus, Krisztina
dc.contributor.author
Wildner, Hendrik
dc.contributor.author
Zeilhofer, Hanns U.
dc.contributor.author
Antal, Miklos
dc.date.accessioned
2022-01-14T10:59:21Z
dc.date.available
2021-08-30T02:43:19Z
dc.date.available
2021-08-30T05:46:54Z
dc.date.available
2022-01-14T10:59:21Z
dc.date.issued
2022-02
dc.identifier.issn
0021-9967
dc.identifier.issn
1096-9861
dc.identifier.issn
0092-7317
dc.identifier.other
10.1002/cne.25232
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/502770
dc.identifier.doi
10.3929/ethz-b-000502770
dc.description.abstract
A growing body of experimental evidence shows that glycinergic inhibition plays vital roles in spinal pain processing. In spite of this, however, our knowledge about the morphology, neurochemical characteristics, and synaptic relations of glycinergic neurons in the spinal dorsal horn is very limited. The lack of this knowledge makes our understanding about the specific contribution of glycinergic neurons to spinal pain processing quite vague. Here we investigated the morphology and neurochemical characteristics of glycinergic neurons in laminae I-IV of the spinal dorsal horn using a GlyT2::CreERT2-tdTomato transgenic mouse line. Confirming previous reports, we show that glycinergic neurons are sparsely distributed in laminae I-II, but their densities are much higher in lamina III and especially in lamina IV. First in the literature, we provide experimental evidence indicating that in addition to neurons in which glycine colocalizes with GABA, there are glycinergic neurons in laminae I-II that do not express GABA and can thus be referred to as glycine-only neurons. According to the shape and size of cell bodies and dendritic morphology, we divided the tdTomato-labeled glycinergic neurons into three and six morphological groups in laminae I-II and laminae III-IV, respectively. We also demonstrate that most of the glycinergic neurons co-express neuronal nitric oxide synthase, parvalbumin, the receptor tyrosine kinase RET, and the retinoic acid-related orphan nuclear receptor beta (ROR beta), but there might be others that need further neurochemical characterization. The present findings may foster our understanding about the contribution of glycinergic inhibition to spinal pain processing.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Wiley
en_US
dc.rights.uri
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject
cell morphology
en_US
dc.subject
glycine transporter 2
en_US
dc.subject
glycinergic neurons
en_US
dc.subject
immunohistochemistry
en_US
dc.subject
mouse
en_US
dc.subject
spinal dorsal horn
en_US
dc.title
Morphological and neurochemical characterization of glycinergic neurons in laminae I-IV of the mouse spinal dorsal horn
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
dc.date.published
2021-08-12
ethz.journal.title
The Journal of Comparative Neurology
ethz.journal.volume
530
en_US
ethz.journal.issue
3
en_US
ethz.journal.abbreviated
J Comp Neurol
ethz.pages.start
607
en_US
ethz.pages.end
626
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
Hoboken, NJ
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02020 - Dep. Chemie und Angewandte Biowiss. / Dep. of Chemistry and Applied Biosc.::02534 - Institut für Pharmazeutische Wiss. / Institute of Pharmaceutical Sciences::03742 - Zeilhofer, Hanns U. / Zeilhofer, Hanns U.
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02020 - Dep. Chemie und Angewandte Biowiss. / Dep. of Chemistry and Applied Biosc.::02534 - Institut für Pharmazeutische Wiss. / Institute of Pharmaceutical Sciences::03742 - Zeilhofer, Hanns U. / Zeilhofer, Hanns U.
ethz.date.deposited
2021-08-30T02:43:34Z
ethz.source
WOS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2022-01-14T10:59:28Z
ethz.rosetta.lastUpdated
2023-02-06T23:49:48Z
ethz.rosetta.versionExported
true
ethz.COinS
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