Imaging of retina cellular and subcellular structures using ptychographic hard X-ray tomography
dc.contributor.author
Panneels, Valerie
dc.contributor.author
Diaz, Ana
dc.contributor.author
Imsand, Cornelia
dc.contributor.author
Guizar-Sicairos, Manuel
dc.contributor.author
Müller, Elisabeth
dc.contributor.author
Bittermann, Anne Greet
dc.contributor.author
Ishikawa, Takashi
dc.contributor.author
Menzel, Andreas
dc.contributor.author
Kaech, Andres
dc.contributor.author
Holler, Mirko
dc.contributor.author
Grimm, Christian
dc.contributor.author
Schertler, Gebhard
dc.date.accessioned
2021-11-08T13:03:28Z
dc.date.available
2021-11-08T13:03:28Z
dc.date.issued
2021-10
dc.identifier.issn
1477-9137
dc.identifier.issn
0021-9533
dc.identifier.other
10.1242/jcs.258561
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/514266
dc.description.abstract
Ptychographic hard X-ray computed tomography (PXCT) is a recent method allowing imaging with quantitative electron-density contrast. Here, we imaged, at cryogenic temperature and without sectioning, cellular and subcellular structures of a chemically fixed and stained wild-type mouse retina, including axons and synapses, with complete isotropic 3D information over tens of microns. Comparison with tomograms of degenerative retina from a mouse model of retinitis pigmentosa illustrates the potential of this method for analyzing disease processes like neurodegeneration at sub-200 nm resolution. As a non-destructive imaging method, PXCT is very suitable for correlative imaging. Within the outer plexiform layer containing the photoreceptor synapses, we identified somatic synapses. We used a small region inside the X-ray-imaged sample for further high-resolution focused ion beam/scanning electron microscope tomography. The subcellular structures of synapses obtained with the X-ray technique matched the electron microscopy data, demonstrating that PXCT is a powerful scanning method for tissue volumes of more than 60 cells and sensitive enough for identification of regions as small as 200 nm, which remain available for further structural and biochemical investigations.
en_US
dc.language.iso
en
en_US
dc.publisher
Company of Biologists
dc.subject
X-ray
en_US
dc.subject
Imaging
en_US
dc.subject
Neurodegeneration
en_US
dc.subject
Retina
en_US
dc.subject
Synapse
en_US
dc.subject
Tomography
en_US
dc.title
Imaging of retina cellular and subcellular structures using ptychographic hard X-ray tomography
en_US
dc.type
Journal Article
dc.date.published
2021-10-14
ethz.journal.title
Journal of Cell Science
ethz.journal.volume
134
en_US
ethz.journal.issue
19
en_US
ethz.journal.abbreviated
J. Cell Sci.
ethz.pages.start
jcs258561
en_US
ethz.size
8 p.
en_US
ethz.identifier.wos
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
Cambridge
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00003 - Schulleitung und Dienste::00022 - Bereich VP Forschung / Domain VP Research::02891 - ScopeM / ScopeM
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00003 - Schulleitung und Dienste::00022 - Bereich VP Forschung / Domain VP Research::02891 - ScopeM / ScopeM
ethz.date.deposited
2021-11-01T03:35:11Z
ethz.source
WOS
ethz.source
SCOPUS
ethz.eth
yes
en_US
ethz.availability
Metadata only
en_US
ethz.rosetta.installDate
2021-11-08T13:03:36Z
ethz.rosetta.lastUpdated
2024-02-02T15:19:47Z
ethz.rosetta.versionExported
true
dc.identifier.olduri
http://hdl.handle.net/20.500.11850/512887
dc.identifier.olduri
http://hdl.handle.net/20.500.11850/512888
dc.identifier.olduri
http://hdl.handle.net/20.500.11850/513315
dc.identifier.olduri
http://hdl.handle.net/20.500.11850/513782
ethz.COinS
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