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dc.contributor.author
Kuett, Laura
dc.contributor.author
Catena, Raúl
dc.contributor.author
Özcan, Alaz
dc.contributor.author
Plüss, Alex
dc.contributor.author
Cancer Grand Challenges IMAXT Consortium
dc.contributor.author
Schraml, Peter
dc.contributor.author
Moch, Holger
dc.contributor.author
de Souza, Natalie
dc.contributor.author
Bodenmiller, Bernd
dc.date.accessioned
2022-01-21T19:49:07Z
dc.date.available
2022-01-01T03:46:46Z
dc.date.available
2022-01-21T19:49:07Z
dc.date.issued
2022-01
dc.identifier.issn
2662-1347
dc.identifier.other
10.1038/s43018-021-00301-w
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/522532
dc.identifier.doi
10.3929/ethz-b-000522532
dc.description.abstract
A holistic understanding of tissue and organ structure and function requires the detection of molecular constituents in their original three-dimensional (3D) context. Imaging mass cytometry (IMC) enables simultaneous detection of up to 40 antigens and transcripts using metal-tagged antibodies but has so far been restricted to two-dimensional imaging. Here we report the development of 3D IMC for multiplexed 3D tissue analysis at single-cell resolution and demonstrate the utility of the technology by analysis of human breast cancer samples. The resulting 3D models reveal cellular and microenvironmental heterogeneity and cell-level tissue organization not detectable in two dimensions. 3D IMC will prove powerful in the study of phenomena occurring in 3D space such as tumor cell invasion and is expected to provide invaluable insights into cellular microenvironments and tissue architecture.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Nature
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.title
Three-dimensional imaging mass cytometry for highly multiplexed molecular and cellular mapping of tissues and the tumor microenvironment
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2021-12-24
ethz.journal.title
Nature Cancer
ethz.journal.volume
3
en_US
ethz.journal.issue
1
en_US
ethz.journal.abbreviated
Nat Cancer
ethz.pages.start
122
en_US
ethz.pages.end
133
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
London
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02539 - Institut für Molecular Health Sciences / Institute of Molecular Health Sciences::09735 - Bodenmiller, Bernd / Bodenmiller, Bernd
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02539 - Institut für Molecular Health Sciences / Institute of Molecular Health Sciences::09735 - Bodenmiller, Bernd / Bodenmiller, Bernd
ethz.date.deposited
2022-01-01T03:47:04Z
ethz.source
WOS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2022-01-21T19:49:36Z
ethz.rosetta.lastUpdated
2023-02-06T23:54:11Z
ethz.rosetta.versionExported
true
ethz.COinS
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