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dc.contributor.author
Schaffhauser, Daniel Felix
dc.contributor.author
Patti, Monica
dc.contributor.author
Goda, Tatsuro
dc.contributor.author
Miyahara, Yuji
dc.contributor.author
Forster, Ian Cameron
dc.contributor.author
Dittrich, Petra S.
dc.date.accessioned
2018-09-13T11:22:41Z
dc.date.available
2017-06-10T03:01:15Z
dc.date.available
2018-09-13T11:22:41Z
dc.date.issued
2012-07-05
dc.identifier.issn
1932-6203
dc.identifier.other
10.1371/journal.pone.0039238
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/52501
dc.identifier.doi
10.3929/ethz-b-000052501
dc.description.abstract
An integrated microdevice for measuring proton-dependent membrane activity at the surface of Xenopus laevis oocytes is presented. By establishing a stable contact between the oocyte vitelline membrane and an ion-sensitive field-effect (ISFET) sensor inside a microperfusion channel, changes in surface pH that are hypothesized to result from facilitated proton lateral diffusion along the membrane were detected. The solute diffusion barrier created between the sensor and the active membrane area allowed detection of surface proton concentration free from interference of solutes in bulk solution. The proposed sensor mechanism was verified by heterologously expressing membrane transport proteins and recording changes in surface pH during application of the specific substrates. Experiments conducted on two families of phosphate-sodium cotransporters (SLC20 & SLC34) demonstrated that it is possible to detect phosphate transport for both electrogenic and electroneutral isoforms and distinguish between transport of different phosphate species. Furthermore, the transport activity of the proton/amino acid cotransporter PAT1 assayed using conventional whole cell electrophysiology correlated well with changes in surface pH, confirming the ability of the system to detect activity proportional to expression level.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Public Library of Science
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/3.0/
dc.title
An Integrated Field-Effect Microdevice for Monitoring Membrane Transport in Xenopus laevis Oocytes via Lateral Proton Diffusion
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 3.0 Unported
ethz.journal.title
PLoS ONE
ethz.journal.volume
7
en_US
ethz.journal.issue
7
en_US
ethz.journal.abbreviated
PLoS ONE
ethz.pages.start
e39238
en_US
ethz.size
8 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.publication.place
Lawrence, KS, USA
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02060 - Dep. Biosysteme / Dep. of Biosystems Science and Eng.::03807 - Dittrich, Petra / Dittrich, Petra
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02060 - Dep. Biosysteme / Dep. of Biosystems Science and Eng.::03807 - Dittrich, Petra / Dittrich, Petra
ethz.date.deposited
2017-06-10T03:02:00Z
ethz.source
ECIT
ethz.identifier.importid
imp59364f7f0d3e125691
ethz.ecitpid
pub:85318
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-14T23:51:20Z
ethz.rosetta.lastUpdated
2021-02-15T01:45:07Z
ethz.rosetta.versionExported
true
ethz.COinS
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