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dc.contributor.author
Abrahams, Jan Pieter
dc.contributor.author
Ban, Nenad
dc.contributor.editor
Carter, Charles W.
dc.contributor.editor
Sweet, Robert M.
dc.date.accessioned
2022-05-05T07:55:48Z
dc.date.available
2017-06-10T03:03:56Z
dc.date.available
2022-05-05T07:55:48Z
dc.date.issued
2003
dc.identifier.isbn
978-0-12-182777-9
en_US
dc.identifier.issn
0076-6879
dc.identifier.other
10.1016/S0076-6879(03)74008-7
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/52546
dc.description.abstract
X-ray crystallography is currently the only technique available that permits high-resolution structure determination of extremely large macromolecular complexes. An increasing number of large structures and macromolecular assemblies have been determined by crystallographic methods. Difference Fourier analysis is powerful in identifying anomalous scatterers or heavy atoms. However, such analysis requires some phase information, and often low-resolution phases are adequate. To determine such low-resolution phases, electron microscopy three-dimensional (3D) reconstructions of the particle should be used. This technique was pioneered in the structure determination of crystals of the tomato bushy stunt virus for which 3D maps derived from electron micrographs of negatively stained virus particles were employed. In that case, the crystallographic symmetry unambiguously determined the orientation and the position of the virus particle in the unit cell, and molecular replacement procedures were not necessary. There has been a dramatic increase in the success of crystallography in solving structures of large asymmetric subunits. The major contributors to this success are the improved quality and flux of synchrotron beam lines that allow data to be measured at the highest possible accuracy.
en_US
dc.language.iso
en
en_US
dc.publisher
Academic Press
en_US
dc.title
X-ray crystallographic structure determination of large asymmetric macromolecular assemblies
en_US
dc.type
Book Chapter
ethz.book.title
Macromolecular Crystallography, Part D
en_US
ethz.journal.title
Methods in Enzymology
ethz.journal.volume
374
en_US
ethz.pages.start
163
en_US
ethz.pages.end
188
en_US
ethz.identifier.wos
ethz.publication.place
New York, NY
en_US
ethz.publication.status
published
en_US
ethz.date.deposited
2017-06-10T03:06:14Z
ethz.source
ECIT
ethz.identifier.importid
imp59364f7fe93e065286
ethz.ecitpid
pub:85364
ethz.eth
yes
en_US
ethz.availability
Metadata only
en_US
ethz.rosetta.installDate
2017-07-12T21:33:48Z
ethz.rosetta.lastUpdated
2020-02-14T10:17:35Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
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