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dc.contributor.author
Nuti, Nicola
dc.contributor.author
Rottmann, Philipp
dc.contributor.author
Stucki, Ariane
dc.contributor.author
Koch, Philipp
dc.contributor.author
Panke, Sven
dc.contributor.author
Dittrich, Petra S.
dc.date.accessioned
2022-01-24T13:45:47Z
dc.date.available
2022-01-17T15:32:50Z
dc.date.available
2022-01-24T13:45:47Z
dc.date.issued
2021-11-19
dc.identifier.other
10.1101/2021.11.17.468707
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/526332
dc.identifier.doi
10.3929/ethz-b-000526332
dc.description.abstract
The global surge in bacterial resistance against traditional antibiotics triggered intensive research for novel compounds, with antimicrobial peptides (AMPs) identified as a promising candidate. Automated methods to systematically generate and screen AMPs according to their membrane preference, however, are still lacking. We introduce a novel microfluidic system for the simultaneous cell-free production and screening of AMPs for their membrane specificity. On our device, AMPs are cell-free produced within water-in-oil-in-water double emulsion droplets, generated at high frequency. Within each droplet, the peptides can interact with different classes of co-encapsulated liposomes, generating a membrane-specific fluorescent signal. The double emulsions can be incubated and observed in a hydrodynamic trapping array or analysed via flow cytometry. Our approach provides a valuable tool for the discovery and development of membrane-active antimicrobials.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Cold Spring Harbor Laboratory
en_US
dc.rights.uri
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject
Analytical methods
en_US
dc.subject
Membranes
en_US
dc.subject
Antimicrobial peptides
en_US
dc.subject
Droplet microfluidics
en_US
dc.subject
Cell-free protein synthesis
en_US
dc.title
A multiplexed cell-free assay to screen for antimicrobial peptides in double emulsion droplets
en_US
dc.type
Working Paper
dc.rights.license
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
ethz.journal.title
bioRxiv
ethz.size
13 p.
en_US
ethz.grant
Engineering of hybrid cells using lab-on-chip technology
en_US
ethz.publication.place
Cold Spring Harbor, NY
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02060 - Dep. Biosysteme / Dep. of Biosystems Science and Eng.::03602 - Panke, Sven / Panke, Sven
en_US
ethz.grant.agreementno
681587
ethz.grant.fundername
EC
ethz.grant.funderDoi
10.13039/501100000780
ethz.grant.program
H2020
ethz.relation.isPreviousVersionOf
10.3929/ethz-b-000526336
ethz.date.deposited
2022-01-17T15:32:56Z
ethz.source
FORM
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2022-01-24T13:45:55Z
ethz.rosetta.lastUpdated
2022-03-29T17:47:33Z
ethz.rosetta.versionExported
true
ethz.COinS
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