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dc.contributor.author
Bellés-Sancho, Paula
dc.contributor.author
Lardi, Martina
dc.contributor.author
Liu, Yilei
dc.contributor.author
Hug, Sebastian
dc.contributor.author
Pinto-Carbó, Marta A.
dc.contributor.author
Zamboni, Nicola
dc.contributor.author
Pessi, Gabriella
dc.date.accessioned
2022-01-19T08:14:14Z
dc.date.available
2022-01-19T08:14:14Z
dc.date.issued
2021-04
dc.identifier.issn
2073-4409
dc.identifier.other
10.3390/cells10040952
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/526767
dc.identifier.doi
10.3929/ethz-b-000484252
dc.description.abstract
Homocitrate is an essential component of the iron-molybdenum cofactor of nitrogenase, the bacterial enzyme that catalyzes the reduction of dinitrogen (N2) to ammonia. In nitrogen-fixing and nodulating alpha-rhizobia, homocitrate is usually provided to bacteroids in root nodules by their plant host. In contrast, non-nodulating free-living diazotrophs encode the homocitrate synthase (NifV) and reduce N2 in nitrogen-limiting free-living conditions. Paraburkholderia phymatum STM815 is a beta-rhizobial strain, which can enter symbiosis with a broad range of legumes, including papilionoids and mimosoids. In contrast to most alpha-rhizobia, which lack nifV, P. phymatum harbors a copy of nifV on its symbiotic plasmid. We show here that P. phymatum nifV is essential for nitrogenase activity both in root nodules of papilionoid plants and in free-living growth conditions. Notably, nifV was dispensable in nodules of Mimosa pudica despite the fact that the gene was highly expressed during symbiosis with all tested papilionoid and mimosoid plants. A metabolome analysis of papilionoid and mimosoid root nodules infected with the P. phymatum wild-type strain revealed that among the approximately 400 measured metabolites, homocitrate and other metabolites involved in lysine biosynthesis and degradation have accumulated in all plant nodules compared to uninfected roots, suggesting an important role of these metabolites during symbiosis.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
MDPI
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
rhizobia
en_US
dc.subject
papilionoid
en_US
dc.subject
mimosoid
en_US
dc.subject
nodule
en_US
dc.subject
gene expression
en_US
dc.subject
metabolomics
en_US
dc.title
Paraburkholderia phymatum Homocitrate Synthase NifV Plays a Key Role for Nitrogenase Activity during Symbiosis with Papilionoids and in Free-Living Growth Conditions
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2021-04-20
ethz.journal.title
Cells
ethz.journal.volume
10
en_US
ethz.journal.issue
4
en_US
ethz.pages.start
952
en_US
ethz.size
20 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
Basel
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02538 - Institut für Molekulare Systembiologie / Institute for Molecular Systems Biology::08839 - Zamboni, Nicola (Tit.-Prof.)
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02538 - Institut für Molekulare Systembiologie / Institute for Molecular Systems Biology::08839 - Zamboni, Nicola (Tit.-Prof.)
ethz.date.deposited
2021-05-17T02:43:45Z
ethz.source
BATCH
ethz.source
SCOPUS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2022-01-19T08:14:23Z
ethz.rosetta.lastUpdated
2022-03-29T17:40:53Z
ethz.rosetta.versionExported
true
dc.identifier.olduri
http://hdl.handle.net/20.500.11850/525504
dc.identifier.olduri
http://hdl.handle.net/20.500.11850/484252
ethz.COinS
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