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dc.contributor.author
Freimoser, Florian M.
dc.contributor.author
Werner, Thomas P.
dc.contributor.author
Amrhein, Nikolaus
dc.date.accessioned
2019-01-24T12:06:41Z
dc.date.available
2017-06-08T16:57:12Z
dc.date.available
2019-01-24T12:06:41Z
dc.date.issued
2007-09-24
dc.identifier.issn
1471-2229
dc.identifier.other
10.1186/1471-2229-7-51
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/5267
dc.identifier.doi
10.3929/ethz-b-000005267
dc.description.abstract
Background Inorganic polyphosphate (poly P), linear chains of phosphate residues linked by energy rich phosphoanhydride bonds, is found in every cell and organelle and is abundant in algae. Depending on its localization and concentration, poly P is involved in various biological functions. It serves, for example, as a phosphate store and buffer against alkali, is involved in energy metabolism and regulates the activity of enzymes. Bacteria defective in poly P synthesis are impaired in biofilm development, motility and pathogenicity. PolyP has also been found in fungal cell walls and bacterial envelopes, but has so far not been measured directly or stained specifically in the cell wall of any plant or alga. Results Here, we demonstrate the presence of poly P in the cell wall of Chlamydomonas reinhardtii by staining with specific poly P binding proteins. The specificity of the poly P signal was verified by various competition experiments, by staining with different poly P binding proteins and by correlation with biochemical quantification. Microscopical investigation at different time-points during growth revealed fluctuations of the poly P signal synchronous with the cell cycle: The poly P staining peaked during late cytokinesis and was independent of the high intracellular poly P content, which fluctuated only slightly during the cell cycle. Conclusion The presented staining method provides a specific and sensitive tool for the study of poly P in the extracellular matrices of algae and could be used to describe the dynamic behaviour of cell wall poly P during the cell cycle. We assume that cell wall poly P and intracellular poly P are regulated by distinct mechanisms and it is suggested that cell wall bound poly P might have important protective functions against toxic compounds or pathogens during cytokinesis, when cells are more vulnerable.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
BioMed Central
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/2.0/
dc.title
Inorganic polyphosphate occurs in the cell wall of Chlamydomonas reinhardtii and accumulates during cytokinesis
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 2.0 Generic
ethz.journal.title
BMC Plant Biology
ethz.journal.volume
7
en_US
ethz.journal.abbreviated
BMC plant biol.
ethz.pages.start
51
en_US
ethz.size
11 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.publication.place
London
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
03252 - Amrhein, Nikolaus
en_US
ethz.leitzahl.certified
03252 - Amrhein, Nikolaus
ethz.leitzahl.certified
03252 - Amrhein, Nikolaus
ethz.date.deposited
2017-06-08T16:57:46Z
ethz.source
ECIT
ethz.identifier.importid
imp59364b8a3192878557
ethz.ecitpid
pub:15527
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-15T15:44:22Z
ethz.rosetta.lastUpdated
2024-02-02T07:02:16Z
ethz.rosetta.versionExported
true
ethz.COinS
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