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dc.contributor.author
Pierantoni, Maria
dc.contributor.author
Le Cann, Sophie
dc.contributor.author
Sotiriou, Vivien
dc.contributor.author
Ahmed, Saima
dc.contributor.author
Bodey, Andrew J.
dc.contributor.author
Jerjen, Iwan
dc.contributor.author
Nowlan, Niamh C.
dc.contributor.author
Isaksson, Hanna
dc.date.accessioned
2022-01-24T15:51:21Z
dc.date.available
2022-01-24T14:02:23Z
dc.date.available
2022-01-24T15:51:21Z
dc.date.issued
2021-04
dc.identifier.issn
8756-3282
dc.identifier.other
10.1016/j.bone.2021.115849
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/527967
dc.identifier.doi
10.3929/ethz-b-000527967
dc.description.abstract
Fetal immobilization affects skeletal development and can lead to severe malformations. Still, how mechanical load affects embryonic bone formation is not fully elucidated. This study combines mechanobiology, image analysis and developmental biology, to investigate the structural effects of muscular loading on embryonic long bones. We present a novel approach involving a semi-automatic workflow, to study the spatial and temporal evolutions of both hard and soft tissues in 3D without any contrast agent at micrometrical resolution. Using high-resolution phase-contrast-enhanced X-ray synchrotron microtomography, we compare the humeri of Splotch-delayed embryonic mice lacking skeletal muscles with healthy littermates. The effects of skeletal muscles on bone formation was studied from the first stages of mineral deposition (Theiler Stages 23 and 24) to just before birth (Theiler Stage 27). The results show that muscle activity affects both growth plate and mineralized regions, especially during early embryonic development. When skeletal muscles were absent, there was reduced mineralization, altered tuberosity size and location, and, at early embryonic stages, decreased chondrocyte density, size and elongation compared to littermate controls. The proposed workflow enhances our understanding of mechanobiology of early bone formation and could be implemented for the study of other complex biological tissues.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Elsevier
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
Mechanobiology
en_US
dc.subject
Growth plate
en_US
dc.subject
Computed tomography
en_US
dc.subject
High-resolution 3D-imaging
en_US
dc.title
Muscular loading affects the 3D structure of both the mineralized rudiment and growth plate at early stages of bone formation
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2021-01-14
ethz.journal.title
Bone
ethz.journal.volume
145
en_US
ethz.pages.start
115849
en_US
ethz.size
13 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
New York, NY
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02140 - Dep. Inf.technologie und Elektrotechnik / Dep. of Inform.Technol. Electrical Eng.::02631 - Institut für Biomedizinische Technik / Institute for Biomedical Engineering::03817 - Stampanoni, Marco F.M. / Stampanoni, Marco F.M.
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02140 - Dep. Inf.technologie und Elektrotechnik / Dep. of Inform.Technol. Electrical Eng.::02631 - Institut für Biomedizinische Technik / Institute for Biomedical Engineering::03817 - Stampanoni, Marco F.M. / Stampanoni, Marco F.M.
en_US
ethz.date.deposited
2022-01-24T14:02:28Z
ethz.source
FORM
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2022-01-24T15:51:29Z
ethz.rosetta.lastUpdated
2022-03-29T17:47:50Z
ethz.rosetta.versionExported
true
ethz.COinS
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