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dc.contributor.author
Gujas, Bojan
dc.contributor.author
Champeyroux, Chloe
dc.contributor.author
Hunkeler, Anna
dc.contributor.author
Robinson, Emilija
dc.contributor.author
Blanco-Touriñán, Noel
dc.contributor.author
Dias Cruz, Tiago Miguel
dc.contributor.author
Van Durme, Matthias
dc.contributor.author
Nowack, Moritz K.
dc.contributor.author
Rodriguez-Villalon, Antia
dc.date.accessioned
2022-02-16T14:42:42Z
dc.date.available
2022-01-24T16:06:46Z
dc.date.available
2022-02-16T14:42:42Z
dc.date.issued
2022-01-20
dc.identifier.other
10.1101/2022.01.18.476728
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/528037
dc.identifier.doi
10.3929/ethz-b-000528037
dc.description.abstract
Membrane fusion and fission are fundamental processes in sustaining cellular compartmentalization. Fission of a lipid bilayer requires a furrow formation that brings membranes in close proximity prior to a contiguous membrane cleavage. Although plant ancestors abandoned cleavage furrow-mediated cytokinesis more than 500 million years ago, here we show that plants still employ this mechanical principle to divide embryonic vacuoles. The evolutionary divergence in PHOSPHATASE AND TENSIN HOMOLOG DELETED ON CHROMOSOME TEN (PTEN) enzymes was required to coordinate this process, as Arabidopsis loss-of-function pten2a pten2b mutants contain hyper compartmentalized embryonic vacuoles. In contrast, PTEN2 overexpression hinders lytic and secretion cellular pathways downstream of TGN in xylem cells. These processes are critical for the formation of secondary cell walls in xylem cells and depend on a poorly characterized and evolutionarily novel N-terminal domain in PTEN2s. The PTEN2 subfamily appeared with the emergence of the Phragmoplastophyta clade, when vacuolar compartments enlarged and cleavage furrow-mediated cytokinesis became extinct. Together, our work suggests that the evolutionary innovation of the PTEN family is conserved across terrestrial plants and central to vacuolar remodelling.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Cold Spring Harbor Laboratory
en_US
dc.rights.uri
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject
Vacuole division
en_US
dc.subject
Cleavage furrow
en_US
dc.subject
Xylem
en_US
dc.subject
Cell differentiation
en_US
dc.subject
PTEN
en_US
dc.title
Evolutionary PTEN gene divergence underpins the remodeling of plant vacuolar compartments
en_US
dc.type
Working Paper
dc.rights.license
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
ethz.journal.title
bioRxiv
ethz.size
53 p.
en_US
ethz.grant
Phosphoinositides- Molecular analysis of new lipid regulators in plant phloem differentiation
en_US
ethz.publication.place
Cold Spring Harbor, NY
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02541 - Institut für Molekulare Pflanzenbiologie / Institute of Molecular Plant Biology::09493 - Rodriguez-Villalon, Antia (ehemalig) / Rodriguez-Villalon, Antia (former)
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02541 - Institut für Molekulare Pflanzenbiologie / Institute of Molecular Plant Biology::09493 - Rodriguez-Villalon, Antia (ehemalig) / Rodriguez-Villalon, Antia (former)
en_US
ethz.grant.agreementno
160201
ethz.grant.fundername
SNF
ethz.grant.funderDoi
10.13039/501100001711
ethz.grant.program
Projekte Lebenswissenschaften
ethz.date.deposited
2022-01-24T16:06:52Z
ethz.source
FORM
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2022-02-16T14:42:49Z
ethz.rosetta.lastUpdated
2022-03-29T18:54:46Z
ethz.rosetta.versionExported
true
ethz.COinS
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