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dc.contributor.author
David, Laure C.
dc.contributor.author
Lee, Sang Kyu
dc.contributor.author
Bruderer, Eduard
dc.contributor.author
Abt, Melanie R.
dc.contributor.author
Fischer-Stettler, Michaela
dc.contributor.author
Tschopp, Marie-Aude
dc.contributor.author
Solhaug, Erik M.
dc.contributor.author
Sanchez, Katarzyna
dc.contributor.author
Zeeman, Samuel C.
dc.date.accessioned
2022-01-26T07:30:39Z
dc.date.available
2022-01-25T15:40:27Z
dc.date.available
2022-01-25T15:41:23Z
dc.date.available
2022-01-26T07:30:39Z
dc.date.issued
2022-01
dc.identifier.issn
0032-0889
dc.identifier.issn
1532-2548
dc.identifier.other
10.1093/plphys/kiab468
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/528504
dc.identifier.doi
10.3929/ethz-b-000528504
dc.description.abstract
b-Amylases (BAMs) are key enzymes of transitory starch degradation in chloroplasts, a process that buffers the availability of photosynthetically fixed carbon over the diel cycle to maintain energy levels and plant growth at night. However, during vascular plant evolution, the BAM gene family diversified, giving rise to isoforms with different compartmentation and bio logical activities. Here, we characterized BETA-AMYLASE 9 (BAM9) of Arabidopsis (Arabidopsis thaliana). Among the BAMs, BAM9 is most closely related to BAM4 but is more widely conserved in plants. BAM9 and BAM4 share features in cluding their plastidial localization and lack of measurable a-1,4-glucan hydrolyzing capacity. BAM4 is a regulator of starch degradation, and bam4 mutants display a starch-excess phenotype. Although bam9 single mutants resemble the wild-type (WT), genetic experiments reveal that the loss of BAM9 markedly enhances the starch-excess phenotypes of mutants al ready impaired in starch degradation. Thus, BAM9 also regulates starch breakdown, but in a different way. Interestingly, BAM9 gene expression is responsive to several environmental changes, while that of BAM4 is not. Furthermore, overexpres sion of BAM9 in the WT reduced leaf starch content, but overexpression in bam4 failed to complement fully that mutant’s starch-excess phenotype, suggesting that BAM9 and BAM4 are not redundant. We propose that BAM9 activates starch degradation, helping to manage carbohydrate availability in response to fluctuations in environmental conditions. As such, BAM9 represents an interesting gene target to explore in crop species.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
American Society of Plant Biologists
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.title
BETA-AMYLASE9 is a plastidial nonenzymatic regulator of leaf starch degradation
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2021-10-18
ethz.journal.title
Plant Physiology
ethz.journal.volume
188
en_US
ethz.journal.issue
1
en_US
ethz.journal.abbreviated
Plant physiol.
ethz.pages.start
191
en_US
ethz.pages.end
207
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
Rockville, MD
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02541 - Institut für Molekulare Pflanzenbiologie / Institute of Molecular Plant Biology::03707 - Zeeman, Samuel C. / Zeeman, Samuel C.
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02541 - Institut für Molekulare Pflanzenbiologie / Institute of Molecular Plant Biology::03707 - Zeeman, Samuel C. / Zeeman, Samuel C.
ethz.date.deposited
2022-01-25T15:40:35Z
ethz.source
FORM
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2022-01-26T07:30:46Z
ethz.rosetta.lastUpdated
2022-03-29T17:50:08Z
ethz.rosetta.versionExported
true
ethz.COinS
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