Show simple item record

dc.contributor.author
Fernandes, Andreia
dc.contributor.author
Hosseini, Vahid
dc.contributor.author
Vogel, Viola
dc.contributor.author
Lovchik, Robert D.
dc.date.accessioned
2022-05-23T06:19:31Z
dc.date.available
2022-01-30T03:48:17Z
dc.date.available
2022-05-23T06:19:31Z
dc.date.issued
2022-01
dc.identifier.issn
1932-6203
dc.identifier.other
10.1371/journal.pone.0262044
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/529764
dc.identifier.doi
10.3929/ethz-b-000529764
dc.description.abstract
Shear stress is extremely important for endothelial cell (EC) function. The popularity of 6- well plates on orbital shakers to impose shear stress on ECs has increased among biologists due to their low cost and simplicity. One characteristic of such a platform is the heterogeneous flow profile within a well. While cells in the periphery are exposed to a laminar and high-velocity pulsatile flow that mimics physiological conditions, the flow in the center is disturbed and imposes low shear stress on the cells, which is characteristic of atheroprone regions. For studies where such heterogeneity is not desired, we present a simple cell-patterning technique to selectively prevent cell growth in the center of the well and facilitate the exclusive collection and analysis of cells in the periphery. This guarantees that cell phenotypes will not be influenced by secreted factors from cells exposed to other shear profiles nor that interesting results are obscured by mixing cells from different regions. We also present a multi-staining platform that compartmentalizes each well into 5 smaller independent regions: Four at the periphery and one in the center. This is ideal for studies that aim to grow cells on the whole well surface, for comparison with previous work and minimal interference in the cell culture, but require screening of markers by immunostaining afterwards. It allows to compare different regions of the well, reduces antibody-related costs, and allows the exploration of multiple markers essential for high-content screening of cell response. By increasing the versatility of the 6-well plate on an orbital shaker system, we hope that these two solutions motivate biologists to pursue studies on EC mechanobiology and beyond.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
PLOS
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.title
Engineering solutions for biological studies of flow-exposed endothelial cells on orbital shakers
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2022-01-21
ethz.journal.title
PLoS ONE
ethz.journal.volume
17
en_US
ethz.journal.issue
1
en_US
ethz.journal.abbreviated
PLoS ONE
ethz.pages.start
e0262044
en_US
ethz.size
11 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.scopus
ethz.publication.place
San Francisco, CA
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02070 - Dep. Gesundheitswiss. und Technologie / Dep. of Health Sciences and Technology::02540 - Institut für Translationale Medizin / Institute of Translational Medicine::03640 - Vogel, Viola / Vogel, Viola
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02070 - Dep. Gesundheitswiss. und Technologie / Dep. of Health Sciences and Technology::02540 - Institut für Translationale Medizin / Institute of Translational Medicine::03640 - Vogel, Viola / Vogel, Viola
ethz.date.deposited
2022-01-30T03:48:25Z
ethz.source
SCOPUS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2022-05-23T06:19:38Z
ethz.rosetta.lastUpdated
2023-02-07T03:12:17Z
ethz.rosetta.versionExported
true
ethz.COinS
ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.atitle=Engineering%20solutions%20for%20biological%20studies%20of%20flow-exposed%20endothelial%20cells%20on%20orbital%20shakers&rft.jtitle=PLoS%20ONE&rft.date=2022-01&rft.volume=17&rft.issue=1&rft.spage=e0262044&rft.issn=1932-6203&rft.au=Fernandes,%20Andreia&Hosseini,%20Vahid&Vogel,%20Viola&Lovchik,%20Robert%20D.&rft.genre=article&rft_id=info:doi/10.1371/journal.pone.0262044&
 Search print copy at ETH Library

Files in this item

Thumbnail

Publication type

Show simple item record