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dc.contributor.author
Heistinger, Lina
dc.contributor.author
Dohm, Juliane C.
dc.contributor.author
Paes, Barbara G.
dc.contributor.author
Koizar, Daniel
dc.contributor.author
Troyer, Christina
dc.contributor.author
Ata, Özge
dc.contributor.author
Steininger-Mairinger, Teresa
dc.contributor.author
Mattanovich, Diethard
dc.date.accessioned
2022-05-09T18:30:52Z
dc.date.available
2022-05-06T17:00:53Z
dc.date.available
2022-05-09T18:30:52Z
dc.date.issued
2022-04-25
dc.identifier.issn
1475-2859
dc.identifier.other
10.1186/s12934-022-01796-3
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/545722
dc.identifier.doi
10.3929/ethz-b-000545722
dc.description.abstract
Background The yeast genus Komagataella currently consists of seven methylotrophic species isolated from tree environments. Well-characterized strains of K. phaffii and K. pastoris are important hosts for biotechnological applications, but the potential of other species from the genus remains largely unexplored. In this study, we characterized 25 natural isolates from all seven described Komagataella species to identify interesting traits and provide a comprehensive overview of the genotypic and phenotypic diversity available within this genus. Results Growth tests on different carbon sources and in the presence of stressors at two different temperatures allowed us to identify strains with differences in tolerance to high pH, high temperature, and growth on xylose. As Komagataella species are generally not considered xylose-utilizing yeasts, xylose assimilation was characterized in detail. Growth assays, enzyme activity measurements and 13C labeling confirmed the ability of K. phaffii to utilize D-xylose via the oxidoreductase pathway. In addition, we performed long-read whole-genome sequencing to generate genome assemblies of all Komagataella species type strains and additional K. phaffii and K. pastoris isolates for comparative analysis. All sequenced genomes have a similar size and share 83–99% average sequence identity. Genome structure analysis showed that K. pastoris and K. ulmi share the same rearrangements in difference to K. phaffii, while the genome structure of K. kurtzmanii is similar to K. phaffii. The genomes of the other, more distant species showed a larger number of structural differences. Moreover, we used the newly assembled genomes to identify putative orthologs of important xylose-related genes in the different Komagataella species. Conclusions By characterizing the phenotypes of 25 natural Komagataella isolates, we could identify strains with improved growth on different relevant carbon sources and stress conditions. Our data on the phenotypic and genotypic diversity will provide the basis for the use of so-far neglected Komagataella strains with interesting characteristics and the elucidation of the genetic determinants of improved growth and stress tolerance for targeted strain improvement.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
BMJ
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
Komagataella species
en_US
dc.subject
Pichia pastoris
en_US
dc.subject
Xylose assimilation
en_US
dc.subject
Yeast diversity
en_US
dc.subject
Genome sequencing
en_US
dc.title
Genotypic and phenotypic diversity among Komagataella species reveals a hidden pathway for xylose utilization
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
ethz.journal.title
Microbial Cell Factories
ethz.journal.volume
21
en_US
ethz.journal.issue
1
en_US
ethz.journal.abbreviated
Microb. cell fact.
ethz.pages.start
70
en_US
ethz.size
16 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.grant
Mechanism of caspase-1 subversion in Salmonella Typhimurium diarrhea
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
London
ethz.publication.status
published
en_US
ethz.grant.agreementno
132997
ethz.grant.fundername
SNF
ethz.grant.funderDoi
10.13039/501100001711
ethz.grant.program
Projektförderung in Biologie und Medizin (Abteilung III)
ethz.date.deposited
2022-05-06T17:01:38Z
ethz.source
WOS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2022-05-09T18:31:28Z
ethz.rosetta.lastUpdated
2023-02-07T02:22:20Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
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