G Protein-coupled Receptor (GPCR) Reconstitution and Labeling for Solution Nuclear Magnetic Resonance (NMR) Studies of the Structural Basis of Transmembrane Signaling
- Review Article
Rights / licenseCreative Commons Attribution 4.0 International
G protein-coupled receptors (GPCRs) are a large membrane protein family found in higher organisms, including the human body. GPCRs mediate cellular responses to diverse extracellular stimuli and thus control key physiological functions, which makes them important targets for drug design. Signaling by GPCRs is related to the structure and dynamics of these proteins, which are modulated by extrinsic ligands as well as by intracellular binding partners such as G proteins and arrestins. Here, we review some basics of using nuclear magnetic resonance (NMR) spectroscopy in solution for the characterization of GPCR conformations and intermolecular interactions that relate to transmembrane signaling. Show more
Journal / seriesMolecules
Pages / Article No.
SubjectG protein-coupled receptors; 19F-NMR; membrane mimetics; stable-isotope labeling; in-membrane chemical modification; amino-acid-specific NMR labeling; sequence-specific NMR labeling
MoreShow all metadata