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dc.contributor.author
Rendueles, Olaya
dc.contributor.author
Ferrières, Lionel
dc.contributor.author
Frétaud, Maxence
dc.contributor.author
Bégaud, Evelyne
dc.contributor.author
Herbomel, Philippe
dc.contributor.author
Levraud, Jean-Pierre
dc.contributor.author
Ghigo, Jean-Marc
dc.date.accessioned
2018-11-27T11:56:29Z
dc.date.available
2017-06-10T08:22:06Z
dc.date.available
2018-11-06T14:21:47Z
dc.date.available
2018-11-27T11:56:29Z
dc.date.issued
2012-07-26
dc.identifier.issn
1553-7374
dc.identifier.issn
1553-7366
dc.identifier.other
10.1371/journal.ppat.1002815
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/54925
dc.identifier.doi
10.3929/ethz-b-000054925
dc.description.abstract
The beneficial contribution of commensal bacteria to host health and homeostasis led to the concept that exogenous non-pathogenic bacteria called probiotics could be used to limit disease caused by pathogens. However, despite recent progress using gnotobiotic mammal and invertebrate models, mechanisms underlying protection afforded by commensal and probiotic bacteria against pathogens remain poorly understood. Here we developed a zebrafish model of controlled co-infection in which germ-free zebrafish raised on axenic living protozoa enabled the study of interactions between host and commensal and pathogenic bacteria. We screened enteric fish pathogens and identified Edwardsiella ictaluri as a virulent strain inducing a strong inflammatory response and rapid mortality in zebrafish larvae infected by the natural oro-intestinal route. Using mortality induced by infection as a phenotypic read-out, we pre-colonized zebrafish larvae with 37 potential probiotic bacterial strains and screened for survival upon E. ictaluri infection. We identified 3 robustly protective strains, including Vibrio parahaemolyticus and 2 Escherichia coli strains. We showed that the observed protective effect of E. coli was not correlated with a reduced host inflammatory response, nor with the release of biocidal molecules by protective bacteria, but rather with the presence of specific adhesion factors such as F pili that promote the emergence of probiotic bacteria in zebrafish larvae. Our study therefore provides new insights into the molecular events underlying the probiotic effect and constitutes a potentially high-throughput in vivo approach to the study of the molecular basis of pathogen exclusion in a relevant model of vertebrate oro-intestinal infection.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Public Library of Science (PLoS)
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/3.0/
dc.title
A New Zebrafish Model of Oro-Intestinal Pathogen Colonization Reveals a Key Role for Adhesion in Protection by Probiotic Bacteria
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 3.0 Unported
ethz.journal.title
PLoS Pathogens
ethz.journal.volume
8
en_US
ethz.journal.issue
7
en_US
ethz.journal.abbreviated
PLoS Pathog
ethz.pages.start
e1002815
en_US
ethz.size
17 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.nebis
005409548
ethz.publication.place
Lawrence, KS
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02720 - Institut für Integrative Biologie / Institute of Integrative Biology::03939 - Velicer, Gregory J. / Velicer, Gregory J.
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02720 - Institut für Integrative Biologie / Institute of Integrative Biology::03939 - Velicer, Gregory J. / Velicer, Gregory J.
ethz.date.deposited
2017-06-10T08:24:32Z
ethz.source
ECIT
ethz.identifier.importid
imp59364fb31d8b285385
ethz.ecitpid
pub:88605
ethz.eth
no
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-24T09:08:18Z
ethz.rosetta.lastUpdated
2019-01-03T12:33:17Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
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