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dc.contributor.author
Vogt, Eva
dc.contributor.author
Field, Christopher
dc.contributor.author
Sonderegger, Lukas
dc.contributor.author
Künzler, Markus
dc.date.accessioned
2022-08-09T12:31:14Z
dc.date.available
2022-06-12T04:00:11Z
dc.date.available
2022-08-09T12:31:14Z
dc.date.issued
2022-04-26
dc.identifier.issn
2160-1836
dc.identifier.other
10.1093/g3journal/jkac095
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/551854
dc.description.abstract
In recent years, a variety of fungal cyclic peptides with interesting bioactivities have been discovered. For many of these peptides, the biosynthetic pathways are unknown and their elucidation often holds surprises. The cyclic and backbone N-methylated omphalotins from Omphalotus olearius were recently shown to constitute a novel class (borosins) of ribosomally synthesized and posttranslationally modified peptides, members of which are produced by many fungi, including species of the genus Rhizopogon. Other recently discovered fungal peptide macrocycles include the mariannamides from Mariannaea elegans and the backbone N-methylated verrucamides and broomeanamides from Myrothecium verrucaria and Sphaerostilbella broomeana, respectively. Here, we present draft genome sequences of four fungal species Rhizopogon roseolus, Mariannaea elegans, Myrothecium verrucaria, and Sphaerostilbella broomeana. We screened these genomes for precursor proteins or gene clusters involved in the mariannamide, verrucamide, and broomeanamide biosynthesis including a general screen for borosin-producing precursor proteins. While our genomic screen for potential ribosomally synthesized and posttranslationally modified peptide precursor proteins of mariannamides, verrucamides, broomeanamides, and borosins remained unsuccessful, antiSMASH predicted nonribosomal peptide synthase gene clusters that may be responsible for the biosynthesis of mariannamides, verrucamides, and broomeanamides. In M. verrucaria, our antiSMASH search led to a putative NRPS gene cluster with a predicted peptide product of 20 amino acids, including multiple nonproteinogenic isovalines. This cluster likely encodes a member of the peptaibols, an antimicrobial class of peptides previously isolated primarily from the Genus Trichoderma. The nonribosomal peptide synthase gene clusters discovered in our screenings are promising candidates for future research.
en_US
dc.language.iso
en
en_US
dc.publisher
Oxford University Press
en_US
dc.subject
ribosomally synthesized and posttranslationally modified peptide
en_US
dc.subject
nonribosomal peptide
en_US
dc.subject
peptaibols
en_US
dc.subject
verrucamides
en_US
dc.subject
mariannamides
en_US
dc.subject
broomeanamides
en_US
dc.subject
Rhizopogon roseolus
en_US
dc.subject
Mariannaea elegans
en_US
dc.subject
Myrothecium verrucaria
en_US
dc.subject
Sphaerostilbella broomeana
en_US
dc.title
Genome sequences of Rhizopogon roseolus, Mariannaea elegans, Myrothecium verrucaria, and Sphaerostilbella broomeana and the identification of biosynthetic gene clusters for fungal peptide natural products
en_US
dc.type
Journal Article
dc.date.published
2022-04-26
ethz.journal.title
G3: Genes, Genomes, Genetics
ethz.journal.volume
12
en_US
ethz.journal.issue
7
en_US
ethz.journal.abbreviated
G3
ethz.pages.start
jkac095
en_US
ethz.size
7 p.
en_US
ethz.grant
Molecular dissection of the chemical defense system of multicellular fungi against predation
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
Oxford
en_US
ethz.publication.status
published
en_US
ethz.grant.agreementno
173097
ethz.grant.fundername
SNF
ethz.grant.funderDoi
10.13039/501100001711
ethz.grant.program
Projekte Lebenswissenschaften
ethz.relation.isCitedBy
10.3929/ethz-b-000573641
ethz.date.deposited
2022-06-12T04:00:27Z
ethz.source
WOS
ethz.eth
yes
en_US
ethz.availability
Metadata only
en_US
ethz.rosetta.installDate
2022-08-09T12:31:21Z
ethz.rosetta.lastUpdated
2022-08-09T12:31:21Z
ethz.rosetta.versionExported
true
ethz.COinS
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