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dc.contributor.author
Faza, Marius B.
dc.contributor.author
Chang, Yiming
dc.contributor.author
Occhipinti, Laura
dc.contributor.author
Kemmler, Stefan
dc.contributor.author
Panse, Vikram G.
dc.date.accessioned
2018-10-25T16:35:54Z
dc.date.available
2017-06-10T09:27:40Z
dc.date.available
2018-10-25T16:35:54Z
dc.date.issued
2012-08-30
dc.identifier.issn
1553-7390
dc.identifier.issn
1553-7404
dc.identifier.other
10.1371/journal.pgen.1002915
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/56213
dc.identifier.doi
10.3929/ethz-b-000056213
dc.description.abstract
Nuclear export of mRNAs and pre-ribosomal subunits (pre40S and pre60S) is fundamental to all eukaryotes. While genetic approaches in budding yeast have identified bona fide export factors for mRNAs and pre60S subunits, little is known regarding nuclear export of pre40S subunits. The yeast heterodimeric transport receptor Mex67-Mtr2 (TAP-p15 in humans) binds mRNAs and pre60S subunits in the nucleus and facilitates their passage through the nuclear pore complex (NPC) into the cytoplasm by interacting with Phe-Gly (FG)-rich nucleoporins that line its transport channel. By exploiting a combination of genetic, cell-biological, and biochemical approaches, we uncovered an unanticipated role of Mex67-Mtr2 in the nuclear export of 40S pre-ribosomes. We show that recruitment of Mex67-Mtr2 to pre40S subunits requires loops emanating from its NTF2-like domains and that the C-terminal FG-rich nucleoporin interacting UBA-like domain within Mex67 contributes to the transport of pre40S subunits to the cytoplasm. Remarkably, the same loops also recruit Mex67-Mtr2 to pre60S subunits and to the Nup84 complex, the respective interactions crucial for nuclear export of pre60S subunits and mRNAs. Thus Mex67-Mtr2 is a unique transport receptor that employs a common interaction surface to participate in the nuclear export of both pre-ribosomal subunits and mRNAs. Mex67-Mtr2 could engage a regulatory crosstalk among the three major export pathways for optimal cellular growth and proliferation.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Public Library of Science (PLoS)
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.title
Role of Mex67-Mtr2 in the Nuclear Export of 40S Pre-Ribosomes
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
ethz.journal.title
PLoS Genetics
ethz.journal.volume
8
en_US
ethz.journal.issue
8
en_US
ethz.journal.abbreviated
PLoS Genet
ethz.pages.start
e1002915
en_US
ethz.size
19 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.grant
Dissecting the biogenesis of eukaryotic ribosomal subunits
en_US
ethz.identifier.wos
ethz.identifier.nebis
005410305
ethz.publication.place
San Francisco, CA
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich, direkt::00012 - Lehre und Forschung, direkt::00007 - Departemente, direkt::02030 - Departement Biologie / Department of Biology::02517 - Institut für Biochemie (IBC) / Institute of Biochemistry (IBC)::03884 - Panse, Vikram G. (SNF-Professur) (ehemalig)
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich, direkt::00012 - Lehre und Forschung, direkt::00007 - Departemente, direkt::02030 - Departement Biologie / Department of Biology::02517 - Institut für Biochemie (IBC) / Institute of Biochemistry (IBC)::03884 - Panse, Vikram G. (SNF-Professur) (ehemalig)
ethz.grant.agreementno
260676
ethz.grant.fundername
EC
ethz.grant.funderDoi
10.13039/501100000780
ethz.grant.program
FP7
ethz.date.deposited
2017-06-10T09:29:33Z
ethz.source
ECIT
ethz.identifier.importid
imp59364fce2cb5338910
ethz.ecitpid
pub:90452
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-12T17:58:22Z
ethz.rosetta.lastUpdated
2018-10-25T16:36:12Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
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