Modification of milk fatty acid composition by feeding forages and agro-industrial byproducts from dry areas to Awassi sheep

Abstract

The study tested the hypothesis that certain underused forages and agro-industrial byproducts available in dry areas may positively influence fatty acid (FA) composition and antioxidative properties of milk by their contents of residual oil or phenolic compounds or both. Sixty multiparous fat-tailed Awassi ewes were allocated to 6 groups in a completely randomized block design. During 50 d, the ewes were group-fed 2.5 kg of dry matter/d per ewe 1 of 6 isonitrogenous and isoenergetic diets (forage:concentrate, 0.3:0.7). The test feeds, comprising 30% of the diets, replaced either barley straw [lentil straw, olive leaves, and Atriplex (saltbush) leaves, rich in phenolic compounds or electrolytes] or conventional concentrate ingredients (olive cake and tomato pomace; ~10% lipids) from the control diet. The diets containing olive cake and tomato pomace were rich in oleic acid (18:1 cis-9; 27% of total dietary FA) and linoleic acid (18:2 cis-9,cis-12; 37%), respectively. Profiles of FA were determined in individual milk samples drawn on d 0 and in wk 1, 3, 5 and 7. Data was analyzed by repeated measurement analysis. No consistent treatment effects on yield and gross nutrient composition of the milk were observed, although some differences occurred. Milk resulting from the Atriplex leaf diet expressed the highest antiradical activity, which was low with control and olive leaves. Feeding the tomato pomace and olive cake diets decreased the proportions of short- and medium-chain FA, whereas oleic acid clearly increased in proportion to total FA. Olive leaves most effectively increased rumenic acid (18:2 cis-9,trans-11) and α-linolenic acid (18:3 cis-9,cis-12,cis-15) in milk fat. This also resulted in the highest α-linolenic acid transfer rate from feed to milk and suggests that olive leaves affect ruminal biohydrogenation at several steps. Several alternative feeds exist with an added value, as they enhance FA with potential health benefits and the stability of the milk with higher antioxidative activity, even though responses to test feeds differed largely. It remains to be investigated whether combinations of these feeds would be complementary in these beneficial effects.