Show simple item record

dc.contributor.author
Casanova, Elisa A.
dc.contributor.author
Okoniewski, Michal J.
dc.contributor.author
Cinelli, Paolo
dc.date.accessioned
2018-09-13T14:22:07Z
dc.date.available
2017-06-10T10:59:58Z
dc.date.available
2018-09-13T14:22:07Z
dc.date.issued
2012-10-15
dc.identifier.issn
1932-6203
dc.identifier.other
10.1371/journal.pone.0047107
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/58436
dc.identifier.doi
10.3929/ethz-b-000058436
dc.description.abstract
The transition between morula and blastocyst stage during preimplantation development represents the first differentiation event of embryogenesis. Morula cells undergo the first cellular specialization and produce two well-defined populations of cells, the trophoblast and the inner cell mass (ICM). Embryonic stem cells (ESCs) with unlimited self-renewal capacity are believed to represent the in vitro counterpart of the ICM. Both mouse and rat ESCs can be derived from the ICM cells, but their in vitro stability differs. In this study we performed a microarray analysis in which we compared the transcriptome of mouse and rat morula, blastocyst, and ICM. This cross-species comparison represents a good model for understanding the differences in derivation and cultivation of ESCs observed in the two species. In order to identify alternative regulation of important molecular mechanisms the investigation of differential gene expression between the two species was extended at the level of signaling pathways, gene families, and single selected genes of interest. Some of the genes differentially expressed between the two species are already known to be important factors in the maintenance of pluripotency in ESCs, like for example Sox2 or Stat3, or play a role in reprogramming somatic cells to pluripotency like c-Myc, Klf4 and p53 and therefore represent interesting candidates to further analyze in vitro in the rat ESCs. This is the first study investigating the gene expression changes during the transition from morula to blastocyst in the rat preimplantation development. Our data show that in the pluripotent pool of cells of the rat and mouse preimplantation embryo substantial differential regulation of genes is present, which might explain the difficulties observed for the derivation and culture of rat ESCs using mouse conditions.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Public Library of Science
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/3.0/
dc.title
Cross-Species Genome Wide Expression Analysis during Pluripotent Cell Determination in Mouse and Rat Preimplantation Embryos
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 3.0 Unported
ethz.journal.title
PLoS ONE
ethz.journal.volume
7
en_US
ethz.journal.issue
10
en_US
ethz.journal.abbreviated
PLoS ONE
ethz.pages.start
e47107
en_US
ethz.size
21 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.nebis
006206116
ethz.publication.place
Lawrence, KS, USA
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00003 - Schulleitung und Dienste::00022 - Bereich VP Forschung / Domain VP Research::02207 - Functional Genomics Center Zurich / Functional Genomics Center Zurich
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00003 - Schulleitung und Dienste::00022 - Bereich VP Forschung / Domain VP Research::02207 - Functional Genomics Center Zurich / Functional Genomics Center Zurich
ethz.date.deposited
2017-06-10T11:02:41Z
ethz.source
ECIT
ethz.identifier.importid
imp59364ffaaa31d21140
ethz.ecitpid
pub:93424
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-15T08:37:49Z
ethz.rosetta.lastUpdated
2021-02-15T01:45:37Z
ethz.rosetta.versionExported
true
ethz.COinS
ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.atitle=Cross-Species%20Genome%20Wide%20Expression%20Analysis%20during%20Pluripotent%20Cell%20Determination%20in%20Mouse%20and%20Rat%20Preimplantation%20Embryos&rft.jtitle=PLoS%20ONE&rft.date=2012-10-15&rft.volume=7&rft.issue=10&rft.spage=e47107&rft.issn=1932-6203&rft.au=Casanova,%20Elisa%20A.&Okoniewski,%20Michal%20J.&Cinelli,%20Paolo&rft.genre=article&rft_id=info:doi/10.1371/journal.pone.0047107&
 Search print copy at ETH Library

Files in this item

Thumbnail

Publication type

Show simple item record