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dc.contributor.author
Subedi, Nikita
dc.contributor.author
Verhagen, Liesbeth Petronella
dc.contributor.author
de Jonge, Paul
dc.contributor.author
Van Eyndhoven, Laura C.
dc.contributor.author
van Turnhout, Mark C.
dc.contributor.author
Koomen, Vera
dc.contributor.author
Baudry, Jean
dc.contributor.author
Eyer, Klaus
dc.contributor.author
Dolstra, Harry
dc.contributor.author
Tel, Jurjen
dc.date.accessioned
2023-04-18T13:31:37Z
dc.date.available
2022-12-29T07:03:22Z
dc.date.available
2023-01-04T15:21:40Z
dc.date.available
2023-04-18T13:31:37Z
dc.date.issued
2023-04
dc.identifier.issn
2701-0198
dc.identifier.other
10.1002/adbi.202200207
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/589375
dc.identifier.doi
10.3929/ethz-b-000589375
dc.description.abstract
Increasing evidence suggests that natural killer (NK) cells are composed of distinct functional subsets. This multifunctional role has made them an attractive choice for anticancer immunotherapy. A functional NK cell repertoire is generated through cellular education, resulting in a heterogeneous NK cell population with distinct capabilities responding to different stimuli. The application of a high-throughput droplet-based microfluidic platform allows monitoring of NK cell-target cell interactions at the single-cell level and in real-time. A variable response of single NK cells toward different target cells is observed, and a distinct population of NK cells (serial killers) capable of inducing multiple target lysis is identified. By assessing the cytotoxic dynamics, it is shown that single umbilical cord blood-derived CD34+ hematopoietic progenitor (HPC)-NK cells display superior antitumor cytotoxicity. With an integrated analysis of cytotoxicity and cytokine secretion, it is shown that target cell interactions augment cytotoxic as well as secretory behavior of NK cells. By providing an integrated assessment of NK cell functions by microfluidics, this study paves the way to further functionally characterize NK cells ultimately aimed to improve cancer immunotherapy.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Wiley-VCH
en_US
dc.rights.uri
http://creativecommons.org/licenses/by-nc/4.0/
dc.subject
droplet-based microfluidics
en_US
dc.subject
effector functions
en_US
dc.subject
functional heterogeneity
en_US
dc.subject
natural killer cells
en_US
dc.subject
NK cell-based immunotherapy
en_US
dc.subject
single cell studies
en_US
dc.title
Single-Cell Profiling Reveals Functional Heterogeneity and Serial Killing in Human Peripheral and Ex Vivo-Generated CD34+Progenitor-Derived Natural Killer Cells
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution-NonCommercial 4.0 International
dc.date.published
2022-12-14
ethz.journal.title
Advanced Biology
ethz.journal.volume
7
en_US
ethz.journal.issue
4
en_US
ethz.journal.abbreviated
Adv. Biology
ethz.pages.start
2200207
en_US
ethz.size
12 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
Weinheim
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02020 - Dep. Chemie und Angewandte Biowiss. / Dep. of Chemistry and Applied Biosc.::02534 - Institut für Pharmazeutische Wiss. / Institute of Pharmaceutical Sciences::09676 - Eyer, Klaus (ehemalig) / Eyer, Klaus (former)
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02020 - Dep. Chemie und Angewandte Biowiss. / Dep. of Chemistry and Applied Biosc.::02534 - Institut für Pharmazeutische Wiss. / Institute of Pharmaceutical Sciences::09676 - Eyer, Klaus (ehemalig) / Eyer, Klaus (former)
ethz.relation.isNewVersionOf
10.3929/ethz-b-000585398
ethz.date.deposited
2022-12-29T07:03:25Z
ethz.source
WOS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2023-04-18T13:32:12Z
ethz.rosetta.lastUpdated
2024-02-02T21:42:18Z
ethz.rosetta.versionExported
true
ethz.COinS
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