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dc.contributor.author
Salathé, Rahel
dc.contributor.author
Tognazzo, Martina
dc.contributor.author
Schmid-Hempel, Regula
dc.contributor.author
Schmid-Hempel, Paul
dc.date.accessioned
2018-09-20T07:35:12Z
dc.date.available
2017-06-10T11:44:38Z
dc.date.available
2018-09-20T07:35:12Z
dc.date.issued
2012-11-14
dc.identifier.issn
1932-6203
dc.identifier.other
10.1371/journal.pone.0049046
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/59580
dc.identifier.doi
10.3929/ethz-b-000059580
dc.description.abstract
We here present an efficient, precise and reliable method to isolate and cultivate healthy and viable single Crithidia bombi cells from bumblebee faeces using flow cytometry. We report a precision of >99% in obtaining single trypanosomatid cells for further culture and analysis (“cloning”). In the study, we have investigated the use of different liquid media to cultivate C. bombi and present an optimal medium for obtaining viable clones from all tested, infected host donors. We show that this method can be applied to genotype a collection of clones from natural infections. Furthermore, we show how to cryo-preserve C. bombi cells to be revived to become infective clones after at least 4 years of storage. Considering the high prevalence of infections in natural populations, our method provides a powerful tool in studying the level and diversity of these infections, and thus enriches the current methodology for the studies of complex host-parasite interactions.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Public Library of Science
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/3.0/
dc.title
Probing Mixed-Genotype Infections I: Extraction and Cloning of Infections from Hosts of the Trypanosomatid Crithidia bombi
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 3.0 Unported
ethz.journal.title
PLoS ONE
ethz.journal.volume
7
en_US
ethz.journal.issue
11
en_US
ethz.journal.abbreviated
PLoS ONE
ethz.pages.start
e49046
en_US
ethz.size
9 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.nebis
006206116
ethz.publication.place
Lawrence, KS, USA
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
03330 - Schmid-Hempel, Paul
en_US
ethz.leitzahl.certified
03330 - Schmid-Hempel, Paul
ethz.date.deposited
2017-06-10T11:44:43Z
ethz.source
ECIT
ethz.identifier.importid
imp59365011ddfb837981
ethz.ecitpid
pub:95151
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-19T08:03:24Z
ethz.rosetta.lastUpdated
2021-02-15T01:48:41Z
ethz.rosetta.versionExported
true
ethz.COinS
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