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dc.contributor.author
Basatvat, Shaghayegh
dc.contributor.author
Bach, Frances C.
dc.contributor.author
Barcellona, Marcos N.
dc.contributor.author
Binch, Abbie L.
dc.contributor.author
Buckley, Conor T.
dc.contributor.author
Bueno, Brian
dc.contributor.author
Chahine, Nadeen O.
dc.contributor.author
Chee, Ana
dc.contributor.author
Creemers, Laura B.
dc.contributor.author
Dudli, Stefan
dc.contributor.author
Fearing, Bailey
dc.contributor.author
Ferguson, Stephen J.
dc.contributor.author
Gansau, Jennifer
dc.contributor.author
Gantenbein, Benjamin
dc.contributor.author
Gawri, Rahul
dc.contributor.author
Glaeser, Juliane D.
dc.contributor.author
Grad, Sibylle
dc.contributor.author
Guerrero, Julien
dc.contributor.author
Haglund, Lisbet
dc.contributor.author
Hernandez, Paula A.
dc.contributor.author
et al.
dc.date.accessioned
2023-03-28T06:28:30Z
dc.date.available
2023-02-05T06:14:28Z
dc.date.available
2023-02-07T09:48:30Z
dc.date.available
2023-03-28T06:28:30Z
dc.date.issued
2023-03
dc.identifier.issn
2572-1143
dc.identifier.other
10.1002/jsp2.1238
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/597090
dc.identifier.doi
10.3929/ethz-b-000597090
dc.description.abstract
Background: In vitro studies using nucleus pulposus (NP) cells are commonly used to investigate disc cell biology and pathogenesis, or to aid in the development of new therapies. However, lab-to-lab variability jeopardizes the much-needed progress in the field. Here, an international group of spine scientists collaborated to standardize extraction and expansion techniques for NP cells to reduce variability, improve comparability between labs and improve utilization of funding and resources.Methods: The most commonly applied methods for NP cell extraction, expansion, and re-differentiation were identified using a questionnaire to research groups worldwide. NP cell extraction methods from rat, rabbit, pig, dog, cow, and human NP tissue were experimentally assessed. Expansion and re-differentiation media and techniques were also investigated.Results: Recommended protocols are provided for extraction, expansion, and re-differentiation of NP cells from common species utilized for NP cell culture.Conclusions: This international, multilab and multispecies study identified cell extraction methods for greater cell yield and fewer gene expression changes by applying species-specific pronase usage, 60-100 U/ml collagenase for shorter durations. Recommendations for NP cell expansion, passage number, and many factors driving successful cell culture in different species are also addressed to support harmonization, rigor, and cross-lab comparisons on NP cells worldwide.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Wiley
en_US
dc.rights.uri
http://creativecommons.org/licenses/by-nc/4.0/
dc.subject
culture
en_US
dc.subject
harmonization
en_US
dc.subject
in vitro
en_US
dc.subject
intervertebral
en_US
dc.subject
nucleus pulposus
en_US
dc.subject
standardization
en_US
dc.title
Harmonization and standardization of nucleus pulposus cell extraction and culture methods
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution-NonCommercial 4.0 International
dc.date.published
2023-01-10
ethz.journal.title
JOR Spine
ethz.journal.volume
6
en_US
ethz.journal.issue
1
en_US
ethz.pages.start
e1238
en_US
ethz.size
42 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
Hoboken, NJ
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02070 - Dep. Gesundheitswiss. und Technologie / Dep. of Health Sciences and Technology::02518 - Institut für Biomechanik / Institute for Biomechanics::03915 - Ferguson, Stephen / Ferguson, Stephen
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02070 - Dep. Gesundheitswiss. und Technologie / Dep. of Health Sciences and Technology::02518 - Institut für Biomechanik / Institute for Biomechanics::03915 - Ferguson, Stephen / Ferguson, Stephen
ethz.date.deposited
2023-02-05T06:14:28Z
ethz.source
WOS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2023-03-28T06:28:32Z
ethz.rosetta.lastUpdated
2024-02-02T21:20:38Z
ethz.rosetta.versionExported
true
ethz.COinS
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