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dc.contributor.author
Potthoff, Eva
dc.contributor.author
Guillaume-Gentil, Orane
dc.contributor.author
Ossola, Dario
dc.contributor.author
Polesel-Maris, Jérôme
dc.contributor.author
LeibundGut-Landmann, Salomé
dc.contributor.author
Zambelli, Tomaso
dc.contributor.author
Vorholt, Julia A.
dc.date.accessioned
2018-07-26T11:29:18Z
dc.date.available
2017-06-10T11:58:32Z
dc.date.available
2018-07-26T11:29:18Z
dc.date.issued
2012-12-20
dc.identifier.issn
1932-6203
dc.identifier.other
10.1371/journal.pone.0052712
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/60158
dc.identifier.doi
10.3929/ethz-b-000060158
dc.description.abstract
Cell adhesion to surfaces represents the basis for niche colonization and survival. Here we establish serial quantification of adhesion forces of different cell types using a single probe. The pace of single-cell force-spectroscopy was accelerated to up to 200 yeast and 20 mammalian cells per probe when replacing the conventional cell trapping cantilever chemistry of atomic force microscopy by underpressure immobilization with fluidic force microscopy (FluidFM). In consequence, statistically relevant data could be recorded in a rapid manner, the spectrum of examinable cells was enlarged, and the cell physiology preserved until approached for force spectroscopy. Adhesion forces of Candida albicans increased from below 4 up to 16 nN at 37°C on hydrophobic surfaces, whereas a Δhgc1-mutant showed forces consistently below 4 nN. Monitoring adhesion of mammalian cells revealed mean adhesion forces of 600 nN of HeLa cells on fibronectin and were one order of magnitude higher than those observed for HEK cells.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Public Library of Science
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/3.0/
dc.title
Rapid and Serial Quantification of Adhesion Forces of Yeast and Mammalian Cells
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 3.0 Unported
ethz.journal.title
PLoS ONE
ethz.journal.volume
7
en_US
ethz.journal.issue
12
en_US
ethz.journal.abbreviated
PLoS ONE
ethz.pages.start
e52712
en_US
ethz.size
9 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.nebis
006206116
ethz.publication.place
Lawrence, Kan.
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
03836 - Leibundgut-Landmann, Salomé (SNF-Professur)
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02520 - Institut für Mikrobiologie / Institute of Microbiology::03740 - Vorholt, Julia / Vorholt, Julia
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02140 - Dep. Inf.technologie und Elektrotechnik / Dep. of Inform.Technol. Electrical Eng.::02631 - Institut für Biomedizinische Technik / Institute for Biomedical Engineering::03741 - Vörös, Janos / Vörös, Janos
en_US
ethz.leitzahl.certified
03836 - Leibundgut-Landmann, Salomé (SNF-Professur)
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02520 - Institut für Mikrobiologie / Institute of Microbiology::03740 - Vorholt, Julia / Vorholt, Julia
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02140 - Dep. Inf.technologie und Elektrotechnik / Dep. of Inform.Technol. Electrical Eng.::02631 - Institut für Biomedizinische Technik / Institute for Biomedical Engineering::03741 - Vörös, Janos / Vörös, Janos
ethz.date.deposited
2017-06-10T11:58:40Z
ethz.source
ECIT
ethz.identifier.importid
imp5936501d7708752058
ethz.ecitpid
pub:96167
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-19T08:54:40Z
ethz.rosetta.lastUpdated
2019-01-02T13:30:16Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
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