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dc.contributor.author
Wolfe, Alan J.
dc.contributor.author
Rademacher, David J.
dc.contributor.author
Roese Mores, Carine
dc.contributor.author
Evans, Robert J.
dc.contributor.author
Overholt, Tyler
dc.contributor.author
Halverson, Thomas
dc.contributor.author
Limeira, Roberto
dc.contributor.author
Matthews, Catherine
dc.contributor.author
Badlani, Gopal
dc.contributor.author
Brubaker, Linda
dc.contributor.author
Walker, Stephen J.
dc.date.accessioned
2023-04-26T09:00:31Z
dc.date.available
2023-04-17T03:21:38Z
dc.date.available
2023-04-26T09:00:31Z
dc.date.issued
2023-05
dc.identifier.issn
0022-5347
dc.identifier.issn
1527-3792
dc.identifier.other
10.1097/JU.0000000000003189
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/608103
dc.description.abstract
Purpose: Interstitial cystitis/bladder pain syndrome is a chronic urological condition diagnosed in nearly 8 million females in the United States. Whether urinary microbiota play an etiological role remains controversial. Most studies assessed the microbiota of interstitial cystitis/bladder pain syndrome patients with voided or catheterized urine as a proxy for bladder urothelium; however, urine may not be a true reflection of the bladder microbiota. Bladder biopsy tissue may provide a more accurate, and thus more clinically relevant, picture of bladder microbiota. Materials and Methods: Bladder biopsy tissues were obtained from: (1) 30 females with interstitial cystitis/bladder pain syndrome (18-80 years old) via cystoscopically guided cold-cup biopsy following therapeutic bladder hydrodistention, and (2) 10 non–interstitial cystitis/bladder pain syndrome females undergoing pelvic organ prolapse repair. To detect bacteria, technical duplicates of each RNAlater-preserved biopsy were subjected to 16S rRNA gene sequencing. To visualize bacteria, paraformaldehyde-fixed, paraffin-embedded biopsies were subjected to a combined multiplexed fluorescence in situ hybridization and fluorescence immunohistochemistry assay and confocal microscopy. Results: Bacteria were detected by 16S rRNA gene sequencing in at least 1 technical duplicate of most biopsies. The most abundant genus was Staphylococcus, followed by Lactobacillus; Escherichia was common but not abundant. There was no significant difference between interstitial cystitis/bladder pain syndrome patients and controls (P > .05). Combined fluorescence in situ hybridization and immunohistochemistry reproducibly detected 16S rRNA in epithelial cells and shed cells in the urothelium and lesioned areas and capillary walls in the lamina propria of human bladder biopsy tissue. Conclusions: We conclude that urothelial and urinary microbiota are similar but not identical in adult females.
en_US
dc.language.iso
en
en_US
dc.publisher
American Urological Association
en_US
dc.subject
16S
en_US
dc.subject
Ribosomal
en_US
dc.subject
RNA
en_US
dc.subject
Urothelium
en_US
dc.subject
Interstitial
en_US
dc.subject
Fluorescence
en_US
dc.subject
Cystitis
en_US
dc.subject
In situ hybridization
en_US
dc.subject
Immunohistochemistry
en_US
dc.title
Detection of Bacteria in Bladder Mucosa of Adult Females
en_US
dc.type
Journal Article
ethz.journal.title
The Journal of Urology
ethz.journal.volume
209
en_US
ethz.journal.issue
5
en_US
ethz.journal.abbreviated
J Urol
ethz.pages.start
937
en_US
ethz.pages.end
949
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
Linthicum, MD
en_US
ethz.publication.status
published
en_US
ethz.date.deposited
2023-04-17T03:21:39Z
ethz.source
SCOPUS
ethz.eth
yes
en_US
ethz.availability
Metadata only
en_US
ethz.rosetta.installDate
2024-02-02T21:45:43Z
ethz.rosetta.lastUpdated
2024-02-02T21:45:43Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
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