Show simple item record

dc.contributor.author
Jiang, Fuze
dc.contributor.author
Liu, Bei
dc.contributor.author
Yue, Yang
dc.contributor.author
Tao, Yile
dc.contributor.author
Xiao, Zhen
dc.contributor.author
Li, Meng
dc.contributor.author
Ji, Zheng
dc.contributor.author
Tang, Jiukai
dc.contributor.author
Qiu, Guangyu
dc.contributor.author
Spillmann, Martin
dc.contributor.author
Cao, Junji
dc.contributor.author
Zhang, Lianjun
dc.contributor.author
Wang, Jing
dc.date.accessioned
2023-09-07T13:11:06Z
dc.date.available
2023-05-28T19:32:47Z
dc.date.available
2023-05-31T13:28:52Z
dc.date.available
2023-09-07T13:11:06Z
dc.date.issued
2023-08-04
dc.identifier.issn
2198-3844
dc.identifier.other
10.1002/advs.202301222
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/614011
dc.identifier.doi
10.3929/ethz-b-000614011
dc.description.abstract
Airborne SARS-CoV-2 virus surveillance faces challenges in complicated biomarker enrichment, interferences from various non-specific matters and extremely low viral load in the urban ambient air, leading to difficulties in detecting SARS-CoV-2 bioaerosols. This work reports a highly specific bioanalysis platform, with an exceptionally low limit-of-detection (≤1 copy m−3) and good analytical accordance with RT-qPCR, relying on surface-mediated electrochemical signaling and enzyme-assisted signal amplification, enabling gene and signal amplification for accurate identification and quantitation of low doses human coronavirus 229E (HCoV-229E) and SARS-CoV-2 viruses in urban ambient air. This work provides a laboratory test using cultivated coronavirus to simulate the airborne spread of SARS-CoV-2, and validate that the platform could reliably detect airborne coronavirus and reveal the transmission characteristics. This bioassay conducts the quantitation of real-world HCoV-229E and SARS-CoV-2 in airborne particulate matters collected from road-side and residential areas in Bern and Zurich (Switzerland) and Wuhan (China), with resultant concentrations verified by RT-qPCR.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Wiley-VCH
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
Bioassay
en_US
dc.subject
Electrochemical biocircuit
en_US
dc.subject
SARS-CoV-2 bioaerosols
en_US
dc.subject
Signal amplification
en_US
dc.subject
Urban ambient air
en_US
dc.title
Direct Quantitation of SARS‐CoV‐2 Virus in Urban Ambient Air via a Continuous‐Flow Electrochemical Bioassay
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2023-05-24
ethz.journal.title
Advanced Science
ethz.journal.volume
10
en_US
ethz.journal.issue
22
en_US
ethz.journal.abbreviated
Adv. Sci.
ethz.pages.start
2301222
en_US
ethz.size
11 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.grant
Development of a real-time biosensing system of SARS-CoV-2 to improve healthcare workers safety during COVID 19 pandemics
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
Weinheim
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02115 - Dep. Bau, Umwelt und Geomatik / Dep. of Civil, Env. and Geomatic Eng.::02608 - Institut für Umweltingenieurwiss. / Institute of Environmental Engineering::03887 - Wang, Jing / Wang, Jing
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02115 - Dep. Bau, Umwelt und Geomatik / Dep. of Civil, Env. and Geomatic Eng.::02608 - Institut für Umweltingenieurwiss. / Institute of Environmental Engineering::03887 - Wang, Jing / Wang, Jing
en_US
ethz.grant.agreementno
198258
ethz.grant.fundername
SNF
ethz.grant.funderDoi
10.13039/501100001711
ethz.grant.program
Special Call on Coronaviruses
ethz.date.deposited
2023-05-28T19:32:47Z
ethz.source
FORM
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2023-09-07T13:11:07Z
ethz.rosetta.lastUpdated
2024-02-03T03:23:39Z
ethz.rosetta.versionExported
true
ethz.COinS
ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.atitle=Direct%20Quantitation%20of%20SARS%E2%80%90CoV%E2%80%902%20Virus%20in%20Urban%20Ambient%20Air%20via%20a%20Continuous%E2%80%90Flow%20Electrochemical%20Bioassay&rft.jtitle=Advanced%20Science&rft.date=2023-08-04&rft.volume=10&rft.issue=22&rft.spage=2301222&rft.issn=2198-3844&rft.au=Jiang,%20Fuze&Liu,%20Bei&Yue,%20Yang&Tao,%20Yile&Xiao,%20Zhen&rft.genre=article&rft_id=info:doi/10.1002/advs.202301222&
 Search print copy at ETH Library

Files in this item

Thumbnail

Publication type

Show simple item record