Show simple item record

dc.contributor.author
Vatandaslar, Hasan
dc.contributor.author
Garzia, Aitor
dc.contributor.author
Meyer, Cindy
dc.contributor.author
Godbersen, Svenja
dc.contributor.author
Brandt, Laura T.L.
dc.contributor.author
Griesbach, Esther
dc.contributor.author
Chao, Jeffrey A.
dc.contributor.author
Tuschl, Thomas
dc.contributor.author
Stoffel, Markus
dc.date.accessioned
2023-06-20T08:08:32Z
dc.date.available
2023-06-18T03:46:28Z
dc.date.available
2023-06-20T08:08:32Z
dc.date.issued
2023-06-09
dc.identifier.issn
2041-1723
dc.identifier.other
10.1038/s41467-023-39135-8
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/617198
dc.identifier.doi
10.3929/ethz-b-000617198
dc.description.abstract
System-wide cross-linking and immunoprecipitation (CLIP) approaches have unveiled regulatory mechanisms of RNA-binding proteins (RBPs) mainly in cultured cells due to limitations in the cross-linking efficiency of tissues. Here, we describe viP-CLIP (in vivo PAR-CLIP), a method capable of identifying RBP targets in mammalian tissues, thereby facilitating the functional analysis of RBP-regulatory networks in vivo. We applied viP-CLIP to mouse livers and identified Insig2 and ApoB as prominent TIAL1 target transcripts, indicating an important role of TIAL1 in cholesterol synthesis and secretion. The functional relevance of these targets was confirmed by showing that TIAL1 influences their translation in hepatocytes. Mutant Tial1 mice exhibit altered cholesterol synthesis, APOB secretion and plasma cholesterol levels. Our results demonstrate that viP-CLIP can identify physiologically relevant RBP targets by finding a factor implicated in the negative feedback regulation of cholesterol biosynthesis.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Nature
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.title
In vivo PAR-CLIP (viP-CLIP) of liver TIAL1 unveils targets regulating cholesterol synthesis and secretion
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
ethz.journal.title
Nature Communications
ethz.journal.volume
14
en_US
ethz.journal.issue
1
en_US
ethz.journal.abbreviated
Nat Commun
ethz.pages.start
3386
en_US
ethz.size
17 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
London
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02539 - Institut für Molecular Health Sciences / Institute of Molecular Health Sciences::03739 - Stoffel, Markus / Stoffel, Markus
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02539 - Institut für Molecular Health Sciences / Institute of Molecular Health Sciences::03739 - Stoffel, Markus / Stoffel, Markus
ethz.date.deposited
2023-06-18T03:46:29Z
ethz.source
SCOPUS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2023-06-20T08:08:33Z
ethz.rosetta.lastUpdated
2024-02-03T00:17:03Z
ethz.rosetta.versionExported
true
ethz.COinS
ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.atitle=In%20vivo%20PAR-CLIP%20(viP-CLIP)%20of%20liver%20TIAL1%20unveils%20targets%20regulating%20cholesterol%20synthesis%20and%20secretion&rft.jtitle=Nature%20Communications&rft.date=2023-06-09&rft.volume=14&rft.issue=1&rft.spage=3386&rft.issn=2041-1723&rft.au=Vatandaslar,%20Hasan&Garzia,%20Aitor&Meyer,%20Cindy&Godbersen,%20Svenja&Brandt,%20Laura%20T.L.&rft.genre=article&rft_id=info:doi/10.1038/s41467-023-39135-8&
 Search print copy at ETH Library

Files in this item

Thumbnail

Publication type

Show simple item record