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dc.contributor.author
Borchers, Sylvia
dc.contributor.author
Bremm, Melanie
dc.contributor.author
Lehrnbecher, Thomas
dc.contributor.author
Dammann, Elke
dc.contributor.author
Pabst, Brigitte
dc.contributor.author
Wölk, Benno
dc.contributor.author
Esser, Ruth
dc.contributor.author
Yildiz, Meral
dc.contributor.author
Eder, Matthias
dc.contributor.author
Stadler, Michael
dc.contributor.author
Bader, Peter
dc.contributor.author
Martin, Hans
dc.contributor.author
Jarisch, Andrea
dc.contributor.author
Schneider, Gisbert
dc.contributor.author
Klingebiel, Thomas
dc.contributor.author
Ganser, Arnold
dc.contributor.author
Weissinger, Eva M.
dc.contributor.author
Koehl, Ulrike
dc.date.accessioned
2018-10-04T09:26:19Z
dc.date.available
2017-06-10T12:43:31Z
dc.date.available
2018-10-04T09:26:19Z
dc.date.issued
2012-12-13
dc.identifier.issn
1932-6203
dc.identifier.other
10.1371/journal.pone.0050248
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/61790
dc.identifier.doi
10.3929/ethz-b-000061790
dc.description.abstract
Background Reconstitution of cytomegalovirus-specific CD3+CD8+ T cells (CMV-CTLs) after allogeneic hematopoietic stem cell transplantation (HSCT) is necessary to bring cytomegalovirus (CMV) reactivation under control. However, the parameters determining protective CMV-CTL reconstitution remain unclear to date. Design and Methods In a prospective tri-center study, CMV-CTL reconstitution was analyzed in the peripheral blood from 278 patients during the year following HSCT using 7 commercially available tetrameric HLA-CMV epitope complexes. All patients included could be monitored with at least CMV-specific tetramer. Results CMV-CTL reconstitution was detected in 198 patients (71%) after allogeneic HSCT. Most importantly, reconstitution with 1 CMV-CTL per µl blood between day +50 and day +75 post-HSCT discriminated between patients with and without CMV reactivation in the R+/D+ patient group, independent of the CMV-epitope recognized. In addition, CMV-CTLs expanded more daramtaically in patients experiencing only one CMV-reactivation than those without or those with multiple CMV reactivations. Monitoring using at least 2 tetramers was possible in 63% (n = 176) of the patients. The combinations of particular HLA molecules influenced the numbers of CMV-CTLs detected. The highest CMV-CTL count obtained for an individual tetramer also changed over time in 11% of these patients (n = 19) resulting in higher levels of HLA-B*0801 (IE-1) recognizing CMV-CTLs in 14 patients. Conclusions Our results indicate that 1 CMV-CTL per µl blood between day +50 to +75 marks the beginning of an immune response against CMV in the R+/D+ group. Detection of CMV-CTL expansion thereafter indicates successful resolution of the CMV reactivation. Thus, sequential monitoring of CMV-CTL reconstitution can be used to predict patients at risk for recurrent CMV reactivation.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Public Library Science
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/3.0/
dc.title
Sequential Anti-Cytomegalovirus Response Monitoring May Allow Prediction of Cytomegalovirus Reactivation after Allogeneic Stem Cell Transplantation
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 3.0 Unported
ethz.journal.title
PLoS ONE
ethz.journal.volume
7
en_US
ethz.journal.issue
12
en_US
ethz.journal.abbreviated
PLoS ONE
ethz.pages.start
e50248
en_US
ethz.size
10 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.nebis
006206116
ethz.publication.place
San Francisco, CA, USA
en_US
ethz.publication.status
published
en_US
ethz.relation.isReferencedBy
10.1371/annotation/43e9b84c-fbe3-4b39-88c8-1cde34b0afea
ethz.date.deposited
2017-06-10T12:45:13Z
ethz.source
ECIT
ethz.identifier.importid
imp5936503a9143f20776
ethz.ecitpid
pub:98400
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-18T07:55:46Z
ethz.rosetta.lastUpdated
2018-10-04T09:27:02Z
ethz.rosetta.versionExported
true
ethz.COinS
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