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dc.contributor.author
Nouri, Sirine
dc.contributor.author
Boudet, Julien
dc.contributor.author
Dreher-Teo, Hiang
dc.contributor.author
Allain, Frédéric H.-T.
dc.contributor.author
Glockshuber, Rudi
dc.contributor.author
Salmon, Loïc
dc.contributor.author
Giese, Christoph
dc.date.accessioned
2023-09-13T08:43:16Z
dc.date.available
2023-07-09T03:43:29Z
dc.date.available
2023-07-10T08:00:37Z
dc.date.available
2023-09-13T08:43:16Z
dc.date.issued
2023-09-11
dc.identifier.issn
1433-7851
dc.identifier.issn
1521-3773
dc.identifier.issn
0570-0833
dc.identifier.other
10.1002/anie.202305120
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/620639
dc.identifier.doi
10.3929/ethz-b-000620639
dc.description.abstract
In NMR spectroscopy, residual dipolar couplings (RDCs) have emerged as one of the most exquisite probes of biological structure and dynamics. The measurement of RDCs relies on the partial alignment of the molecule of interest, for example by using a liquid crystal as a solvent. Here, we establish bacterial type 1 pili as an alternative liquid-crystalline alignment medium for the measurement of RDCs. To achieve alignment at pilus concentrations that allow for efficient NMR sample preparation, we elongated wild-type pili by recombinant overproduction of the main structural pilus subunit. Building on the extraordinary stability of type 1 pili against spontaneous dissociation and unfolding, we show that the medium is compatible with challenging experimental conditions such as high temperature, the presence of detergents, organic solvents or very acidic pH, setting it apart from most established alignment media. Using human ubiquitin, HIV-1 TAR RNA and camphor as spectroscopic probes, we demonstrate the applicability of the medium for the determination of RDCs of proteins, nucleic acids and small molecules. Our results show that type 1 pili represent a very useful alternative to existing alignment media and may readily assist the characterization of molecular structure and dynamics by NMR.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Wiley-VCH
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
Alignment Medium
en_US
dc.subject
Liquid Crystals
en_US
dc.subject
NMR Spectroscopy
en_US
dc.subject
Residual Dipolar Couplings
en_US
dc.subject
Type 1 Pili
en_US
dc.title
Elongated Bacterial Pili as a Versatile Alignment Medium for NMR Spectroscopy
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2023-05-29
ethz.journal.title
Angewandte Chemie. International Edition
ethz.journal.volume
62
en_US
ethz.journal.issue
37
en_US
ethz.journal.abbreviated
Angew. Chem. Int. Ed.
ethz.pages.start
e202305120
en_US
ethz.size
7 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
Weinheim
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02517 - Institut für Biochemie / Institute of Biochemistry (IBC)::03591 - Allain, Frédéric / Allain, Frédéric
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02521 - Inst. f. Molekularbiologie u. Biophysik / Inst. Molecular Biology and Biophysics::03412 - Glockshuber, Rudolf / Glockshuber, Rudolf
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02517 - Institut für Biochemie / Institute of Biochemistry (IBC)::03591 - Allain, Frédéric / Allain, Frédéric
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02521 - Inst. f. Molekularbiologie u. Biophysik / Inst. Molecular Biology and Biophysics::03412 - Glockshuber, Rudolf / Glockshuber, Rudolf
ethz.date.deposited
2023-07-09T03:43:34Z
ethz.source
WOS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2023-09-13T08:43:17Z
ethz.rosetta.lastUpdated
2024-02-03T03:27:46Z
ethz.rosetta.versionExported
true
ethz.COinS
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