Show simple item record

dc.contributor.author
Cooksley-Decasper, Seraina
dc.contributor.author
Reiser, Hans
dc.contributor.author
Thommen, Daniela S.
dc.contributor.author
Biedermann, Barbara
dc.contributor.author
Neidhart, Michel
dc.contributor.author
Gawinecka, Johanna
dc.contributor.author
Cathomas, Gieri
dc.contributor.author
Franzeck, Fabian C.
dc.contributor.author
Wyss, Christoph
dc.contributor.author
Klingenberg, Roland
dc.contributor.author
Nanni, Paolo
dc.contributor.author
Roschitzki, Bernd
dc.contributor.author
Matter, Christian
dc.contributor.author
Wolint, Petra
dc.contributor.author
Emmert, Maximilian Y.
dc.contributor.author
Husmann, Marc
dc.contributor.author
Amann-Vesti, Beatrice
dc.contributor.author
Maier, Wilibald
dc.contributor.author
Gay, Steffen
dc.contributor.author
Lüscher, Thomas F.
dc.contributor.author
von Eckardstein, Arnold
dc.contributor.author
Hof, Danielle
dc.date.accessioned
2018-12-06T10:46:32Z
dc.date.available
2017-06-10T12:59:01Z
dc.date.available
2018-12-06T10:46:32Z
dc.date.issued
2012-10-24
dc.identifier.issn
1932-6203
dc.identifier.other
10.1371/journal.pone.0047985
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/62160
dc.identifier.doi
10.3929/ethz-b-000062160
dc.description.abstract
To date, no plaque-derived blood biomarker is available to allow diagnosis, prognosis or monitoring of atherosclerotic vascular diseases. In this study, specimens of thrombendarterectomy material from carotid and iliac arteries were incubated in protein-free medium to obtain plaque and control secretomes for subsequent subtractive phage display. The selection of nine plaque secretome-specific antibodies and the analysis of their immunopurified antigens by mass spectrometry led to the identification of 22 proteins. One of them, junction plakoglobin (JUP-81) and its smaller isoforms (referred to as JUP-63, JUP-55 and JUP-30 by molecular weight) were confirmed by immunohistochemistry and immunoblotting with independent antibodies to be present in atherosclerotic plaques and their secretomes, coronary thrombi of patients with acute coronary syndrome (ACS) and macrophages differentiated from peripheral blood monocytes as well as macrophage-like cells differentiated from THP1 cells. Plasma of patients with stable coronary artery disease (CAD) (n = 15) and ACS (n = 11) contained JUP-81 at more than 2- and 14-fold higher median concentrations, respectively, than plasma of CAD-free individuals (n = 13). In conclusion, this proof of principle study identified and verified JUP isoforms as potential plasma biomarkers for atherosclerosis. Clinical validation studies are needed to determine its diagnostic efficacy and clinical utility as a biomarker for diagnosis, prognosis or monitoring of atherosclerotic vascular diseases.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Public Library of Science
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/3.0/
dc.title
Antibody Phage Display Assisted Identification of Junction Plakoglobin as a Potential Biomarker for Atherosclerosis
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 3.0 Unported
ethz.journal.title
PLoS ONE
ethz.journal.volume
7
en_US
ethz.journal.issue
10
en_US
ethz.journal.abbreviated
PLoS ONE
ethz.pages.start
e47985
en_US
ethz.size
13 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.nebis
006206116
ethz.publication.place
Lawrence, KS, USA
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00003 - Schulleitung und Dienste::00022 - Bereich VP Forschung & Wirtschaftsbez. / Domain VP Research & Corporate Relations::02207 - Functional Genomics Center Zürich / Functional Genomics Center Zürich
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00003 - Schulleitung und Dienste::00022 - Bereich VP Forschung & Wirtschaftsbez. / Domain VP Research & Corporate Relations::02207 - Functional Genomics Center Zürich / Functional Genomics Center Zürich
ethz.date.deposited
2017-06-10T13:01:02Z
ethz.source
ECIT
ethz.identifier.importid
imp593650407330540507
ethz.ecitpid
pub:98824
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-13T08:18:45Z
ethz.rosetta.lastUpdated
2018-12-06T10:46:38Z
ethz.rosetta.versionExported
true
ethz.COinS
ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.atitle=Antibody%20Phage%20Display%20Assisted%20Identification%20of%20Junction%20Plakoglobin%20as%20a%20Potential%20Biomarker%20for%20Atherosclerosis&rft.jtitle=PLoS%20ONE&rft.date=2012-10-24&rft.volume=7&rft.issue=10&rft.spage=e47985&rft.issn=1932-6203&rft.au=Cooksley-Decasper,%20Seraina&Reiser,%20Hans&Thommen,%20Daniela%20S.&Biedermann,%20Barbara&Neidhart,%20Michel&rft.genre=article&
 Search via SFX

Files in this item

Thumbnail

Publication type

Show simple item record