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dc.contributor.author
Tiefenauer, Louis
dc.contributor.author
Demarche, Sophie
dc.date.accessioned
2019-10-01T13:45:24Z
dc.date.available
2017-06-10T13:03:48Z
dc.date.available
2019-10-01T13:45:24Z
dc.date.issued
2012-11-07
dc.identifier.issn
1996-1944
dc.identifier.other
10.3390/ma5112205
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/62268
dc.identifier.doi
10.3929/ethz-b-000062268
dc.description.abstract
Lipid bilayers are natural barriers of biological cells and cellular compartments. Membrane proteins integrated in biological membranes enable vital cell functions such as signal transduction and the transport of ions or small molecules. In order to determine the activity of a protein of interest at defined conditions, the membrane protein has to be integrated into artificial lipid bilayers immobilized on a surface. For the fabrication of such biosensors expertise is required in material science, surface and analytical chemistry, molecular biology and biotechnology. Specifically, techniques are needed for structuring surfaces in the micro- and nanometer scale, chemical modification and analysis, lipid bilayer formation, protein expression, purification and solubilization, and most importantly, protein integration into engineered lipid bilayers. Electrochemical and optical methods are suitable to detect membrane activity-related signals. The importance of structural knowledge to understand membrane protein function is obvious. Presently only a few structures of membrane proteins are solved at atomic resolution. Functional assays together with known structures of individual membrane proteins will contribute to a better understanding of vital biological processes occurring at biological membranes. Such assays will be utilized in the discovery of drugs, since membrane proteins are major drug targets
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
MDPI
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/3.0/
dc.subject
Membrane protein
en_US
dc.subject
Function
en_US
dc.subject
Surface
en_US
dc.subject
Lipid bilayers
en_US
dc.subject
Silicon
en_US
dc.subject
Polymer
en_US
dc.subject
Microfluidics
en_US
dc.subject
Review
en_US
dc.title
Challenges in the Development of Functional Assays of Membrane Proteins
en_US
dc.type
Review Article
dc.rights.license
Creative Commons Attribution 3.0 Unported
ethz.journal.title
Materials
ethz.journal.volume
5
en_US
ethz.journal.issue
11
en_US
ethz.pages.start
2205
en_US
ethz.pages.end
2242
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.nebis
005826344
ethz.publication.place
Basel
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::03866 - Schertler, Gebhard F. / Schertler, Gebhard F.
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::03866 - Schertler, Gebhard F. / Schertler, Gebhard F.
ethz.date.deposited
2017-06-10T13:05:12Z
ethz.source
ECIT
ethz.identifier.importid
imp5936504207c1d16909
ethz.ecitpid
pub:98942
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-20T13:46:08Z
ethz.rosetta.lastUpdated
2020-02-15T21:51:20Z
ethz.rosetta.versionExported
true
ethz.COinS
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