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dc.contributor.author
Karlsson, Maria
dc.contributor.author
Weber, Wilfried
dc.contributor.author
Fussenegger, Martin
dc.contributor.editor
Weber, Wilfried
dc.contributor.editor
Fussenegger, Martin
dc.date.accessioned
2021-07-12T11:16:16Z
dc.date.available
2017-06-10T13:08:40Z
dc.date.available
2021-07-12T10:49:01Z
dc.date.available
2021-07-12T11:16:16Z
dc.date.issued
2012
dc.identifier.isbn
978-1-61779-411-7
en_US
dc.identifier.isbn
978-1-61779-412-4
en_US
dc.identifier.issn
1064-3745
dc.identifier.issn
1940-6029
dc.identifier.other
10.1007/978-1-61779-412-4_22
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/62412
dc.description.abstract
Advances in the development of molecular tools for the inducible control of transcription, translation, and protein degradation are the basis for the rapidly emerging design and construction of synthetic gene net works in mammalian cells. In this chapter, we describe such tools and how they can be integrated into a synthetic gene network with desired functionality. The network design and construction process is illustrated in the form of a detailed protocol for the implementation of a logic NOR gate based on an inducible promoter combined with an inducible protein degradation system.
en_US
dc.language.iso
en
en_US
dc.publisher
Humana Press
en_US
dc.subject
Auxin
en_US
dc.subject
Boolean logic
en_US
dc.subject
Degron
en_US
dc.subject
Inducible expression
en_US
dc.subject
NOR gate
en_US
dc.title
Design and construction of synthetic gene networks in mammalian cells
en_US
dc.type
Book Chapter
dc.date.published
2011-10-14
ethz.book.title
Synthetic Gene Networks
en_US
ethz.journal.title
Methods in Molecular Biology
ethz.journal.volume
813
en_US
ethz.journal.abbreviated
Methods Mol Biol
ethz.pages.start
359
en_US
ethz.pages.end
376
en_US
ethz.identifier.nebis
000916990
ethz.publication.place
New York, NY
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02060 - Dep. Biosysteme / Dep. of Biosystems Science and Eng.::03694 - Fussenegger, Martin / Fussenegger, Martin
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02060 - Dep. Biosysteme / Dep. of Biosystems Science and Eng.::03694 - Fussenegger, Martin / Fussenegger, Martin
ethz.relation.isPartOf
handle/20.500.11850/494552
ethz.date.deposited
2017-06-10T13:08:47Z
ethz.source
ECIT
ethz.identifier.importid
imp593650446cf9895096
ethz.ecitpid
pub:99103
ethz.eth
yes
en_US
ethz.availability
Metadata only
en_US
ethz.rosetta.installDate
2017-07-14T23:23:57Z
ethz.rosetta.lastUpdated
2021-02-14T09:58:11Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
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