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dc.contributor.author
Strotton, Merrick
dc.contributor.author
Hosogane, Tsuyoshi
dc.contributor.author
di Michiel, Marco
dc.contributor.author
Moch, Holger
dc.contributor.author
Varga, Zsuzsanna
dc.contributor.author
Bodenmiller, Bernd
dc.date.accessioned
2023-09-13T08:13:07Z
dc.date.available
2023-09-13T03:23:56Z
dc.date.available
2023-09-13T08:13:07Z
dc.date.issued
2023-09
dc.identifier.issn
1548-7105
dc.identifier.issn
1548-7091
dc.identifier.other
10.1038/s41592-023-01977-x
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/631094
dc.identifier.doi
10.3929/ethz-b-000631094
dc.description.abstract
Rapid, highly multiplexed, nondestructive imaging that spans the molecular to the supra-cellular scale would be a powerful tool for tissue analysis. However, the physical constraints of established imaging methods limit the simultaneous improvement of these parameters. Whole-organism to atomic-level imaging is possible with tissue-penetrant, picometer-wavelength X-rays. To enable highly multiplexed X-ray imaging, we developed multielement Z-tag X-ray fluorescence (MEZ-XRF) that can operate at kHz speeds when combined with signal amplification by exchange reaction (SABER)-amplified Z-tag reagents. We demonstrated parallel imaging of 20 Z-tag or SABER Z-tag reagents at subcellular resolution in cell lines and multiple human tissues. We benchmarked MEZ-XRF against imaging mass cytometry and demonstrated the nondestructive multiscale repeat imaging capabilities of MEZ-XRF with rapid tissue overview scans, followed by slower, more sensitive imaging of low-abundance markers such as immune checkpoint proteins. The unique multiscale, nondestructive nature of MEZ-XRF, combined with SABER Z-tags for high sensitivity or enhanced speed, enables highly multiplexed bioimaging across biological scales.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Nature
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.title
Multielement Z-tag imaging by X-ray fluorescence microscopy for next-generation multiplex imaging
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2023-08-31
ethz.journal.title
Nature Methods
ethz.journal.volume
20
en_US
ethz.journal.issue
9
en_US
ethz.journal.abbreviated
Nat Methods
ethz.pages.start
1310
en_US
ethz.pages.end
1322
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
New York, NY
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02539 - Institut für Molecular Health Sciences / Institute of Molecular Health Sciences::09735 - Bodenmiller, Bernd / Bodenmiller, Bernd
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02539 - Institut für Molecular Health Sciences / Institute of Molecular Health Sciences::09735 - Bodenmiller, Bernd / Bodenmiller, Bernd
ethz.date.deposited
2023-09-13T03:23:58Z
ethz.source
SCOPUS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2023-09-13T08:13:09Z
ethz.rosetta.lastUpdated
2024-02-03T03:27:36Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
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