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dc.contributor.author
Sanz-Sáez, Isabel
dc.contributor.author
Sánchez, Pablo
dc.contributor.author
Salazar Guiral, Guillem
dc.contributor.author
Sunagawa, Shinichi
dc.contributor.author
de Vargas, Colomban
dc.contributor.author
Bowler, Chris
dc.contributor.author
Sullivan, Matthew B.
dc.contributor.author
Wincker, Patrick
dc.contributor.author
Karsenti, Eric
dc.contributor.author
Pedrós-Alió, Carlos
dc.contributor.author
Agustí, Susana
dc.contributor.author
Gojobori, Takashi
dc.contributor.author
Duarte, Carlos M.
dc.contributor.author
Gasol, Josep M.
dc.contributor.author
Sánchez, Olga
dc.contributor.author
Acinas, Silvia G.
dc.date.accessioned
2023-09-18T09:19:24Z
dc.date.available
2023-09-16T03:05:00Z
dc.date.available
2023-09-18T09:19:24Z
dc.date.issued
2023
dc.identifier.issn
2730-6151
dc.identifier.other
10.1038/s43705-023-00290-0
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/631804
dc.identifier.doi
10.3929/ethz-b-000631804
dc.description.abstract
Traditional culture techniques usually retrieve a small fraction of the marine microbial diversity, which mainly belong to the so-called rare biosphere. However, this paradigm has not been fully tested at a broad scale, especially in the deep ocean. Here, we examined the fraction of heterotrophic bacterial communities in photic and deep ocean layers that could be recovered by culture-dependent techniques at a large scale. We compared 16S rRNA gene sequences from a collection of 2003 cultured heterotrophic marine bacteria with global 16S rRNA metabarcoding datasets (16S TAGs) covering surface, mesopelagic and bathypelagic ocean samples that included 16 of the 23 samples used for isolation. These global datasets represent 60 322 unique 16S amplicon sequence variants (ASVs). Our results reveal a significantly higher proportion of isolates identical to ASVs in deeper ocean layers reaching up to 28% of the 16S TAGs of the bathypelagic microbial communities, which included the isolation of 3 of the top 10 most abundant 16S ASVs in the global bathypelagic ocean, related to the genera Sulfitobacter, Halomonas and Erythrobacter. These isolates contributed differently to the prokaryotic communities across different plankton size fractions, recruiting between 38% in the free-living fraction (0.2-0.8 & mu;m) and up to 45% in the largest particles (20-200 & mu;m) in the bathypelagic ocean. Our findings support the hypothesis that sinking particles in the bathypelagic act as resource-rich habitats, suitable for the growth of heterotrophic bacteria with a copiotroph lifestyle that can be cultured, and that these cultivable bacteria can also thrive as free-living bacteria.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Springer
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.title
Top abundant deep ocean heterotrophic bacteria can be retrieved by cultivation
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2023-09-02
ethz.journal.title
ISME Communications
ethz.journal.volume
3
en_US
ethz.journal.abbreviated
ISME COMMUN.
ethz.pages.start
92
en_US
ethz.size
13 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.grant
Resolving ocean microbial micro-diversity and its environmental drivers at global scale
en_US
ethz.identifier.wos
ethz.publication.place
London
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02520 - Institut für Mikrobiologie / Institute of Microbiology::09583 - Sunagawa, Shinichi / Sunagawa, Shinichi
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02520 - Institut für Mikrobiologie / Institute of Microbiology::09583 - Sunagawa, Shinichi / Sunagawa, Shinichi
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02520 - Institut für Mikrobiologie / Institute of Microbiology::09583 - Sunagawa, Shinichi / Sunagawa, Shinichi
ethz.grant.agreementno
184955
ethz.grant.fundername
SNF
ethz.grant.funderDoi
10.13039/501100001711
ethz.grant.program
Projekte MINT
ethz.date.deposited
2023-09-16T03:05:01Z
ethz.source
WOS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2023-09-18T09:19:25Z
ethz.rosetta.lastUpdated
2024-02-03T03:35:58Z
ethz.rosetta.versionExported
true
ethz.COinS
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